Protein Gel and Imaging Analysis Service

Gel electrophoresis is an important protein analysis method, which includes multiple techniques such as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). SDS-PAGE is the most commonly used technique in protein analysis, providing a simple way to estimate the molecular weight of proteins, evaluate sample complexity or preparation purity, and monitor products obtained during chromatography or other purification processes. Additionally, ICH Q6B requires electrophoresis profiles for biopharmaceutical analysis.

 

Services at MtoZ Biolabs

Gel electrophoresis has been used in protein research for many years, separating proteins based on differing physicochemical parameters. MtoZ Biolabs provides a variety of protein gel and image analysis services.

 

1. Protein Separation Based on SDS-PAGE

SDS-PAGE is often used in protein expression analysis. It can provide electrophoresis profiles of protein samples and analyze or purify samples in combination with other mass spectrometry-based protein identification services for proteomic analysis of complex biological samples.

 

2. 1D SDS-PAGE and IEF Services

1D SDS-PAGE separates proteins based on differences in molecular weight. SDS-PAGE is widely used in protein analysis, including the determination of protein size, protein identification, and sample purity analysis.

 

3. 2-DE Services

Two-dimensional electrophoresis (2-DE) is a method to separate complex protein mixtures. 2-DE first uses IEF, an electrophoresis technique for protein separation based on isoelectric points (pI), to separate proteins with similar sizes. SDS-PAGE is then used for further separation. MtoZ Biolabs provides 2-DE analysis services. High-resolution 2-DE can analyze protein species and subtype distribution, host cell protein analysis, and quantitative comparisons of cellular states.

 

4. 2D BN/SDS-PAGE Complex Analysis Services

In biomembranes, many proteins are composed in complexes. MtoZ Biolabs can comprehensively analyze the subunits of these protein complexes using 2D Blue Native (BN)/SDS-PAGE. In 2D BN/SDS-PAGE analysis, samples are separated in the 1D by blue native polyacrylamide gel electrophoresis (BN-PAGE) and then in the 2D using SDS-PAGE.

 

5. WB and Electrotransfer Services

Western blot (WB) is another commonly used protein analysis method for qualitative and semi-quantitative protein analysis. In this method, proteins are first separated by 2-DE and then transferred onto a carrier membrane through electrotransfer. WB can be used for qualitative detection of individual proteins and protein modifications.

 

Service Advantages

1. Compatible with Multiple Techniques, Including Molecular Weight Measurement, Impurity Characterization, Protein Expression Research, etc.

2. Suitable for Various Sample Types, Including Body Fluids, Tissues, Blood Samples, Cell Cultures, Microorganisms, Plants, etc.

3. Multiple Gel Size Options, Including 8×7 cm, 23×30 cm, 40×30 cm, and 60×30 cm

4. Experienced Technicians can Provide Diverse, Customizable Protein Gel and Image Analysis Services

• • 1D SDS-PAGE and IEF Services

  Polyacrylamide gel electrophoresis (PAGE) is a widely used technique for separating macromolecules, including DNA, RNA, and proteins. In this process, charged ions migrate under an electric field. The mobility of charged molecules is proportional to their net charge and the resistance of the solution they pass through. Sodium dodecyl sulfate (SDS), an anionic detergent, imparts a net negative charge to polypeptide chains over a broad pH range.

• • Two-Dimensional Gel Electrophoresis Service

  Two-dimensional gel electrophoresis (2-DE), also known as two-dimensional electrophoresis, is a powerful and widely used method. Two-dimensional gel electrophoresis combines isoelectric focusing and SDS-PAGE. First, proteins are separated by their isoelectric point (pI) in isoelectric focusing, and then by SDS-PAGE based on molecular weight. Each protein spot on the two-dimensional gel can be eluted and identified by high-throughput mass spectrometry.

• • Two-Dimensional Gel Electrophoresis Images Analysis Service

  The most demanding and crucial part of 2D gel electrophoresis technology is the analysis of the resulting protein images. The image data are compared and analyzed automatically. Comparative analysis of 2D gel images can reveal qualitative and/or quantitative changes in protein expression between individual samples or different groups.

• • 2D Blue Native/SDS-PAGE Protein Complex Analysis Service

  In biological membranes, many proteins form complexes. MtoZ Biolabs provides comprehensive subunit analysis of these protein complexes using 2D blue native (BN)/SDS-PAGE. In 2D BN/SDS-PAGE analysis, samples undergo 1D separation through BN-PAGE, followed by 2D separation via SDS-PAGE.

• • Protein Immunoblotting and Electrotransfer Services

  The process where proteins separated by electrophoresis are transferred or “blotted” from the gel matrix onto a membrane (usually nitrocellulose or polyvinylidene difluoride (PVDF)), followed by subsequent antibody-based detection on the membrane surface, is known as western blot (WB) or immunoblotting. Mtoz Biolabs provides WB analysis services.

• • SDS-PAGE Based Protein Separation Service

  Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is frequently employed in protein expression analysis and other scientific research endeavors. It facilitates obtaining electrophoretic profiles of protein samples, which, when combined with mass spectrometry-based protein identification services, allows for analysis or purification of samples, particularly for proteomic analysis of complex biological samples.