Protein Isoelectric Point Determination Service
Proteins are ampholytes that contain both positively and negatively charged functional groups. Their net charge is predominantly determined by the pH of the surrounding environment. When the pH of the external environment makes the acidic and alkaline dissociations in the protein molecule balance, i.e., the positive and negative charges carried by the molecule are completely canceled out, this particular pH is known as the isoelectric point (pI) of that protein. At the pI, the protein's surface charge is zero, resulting in minimal solubility. The pI is specific to each protein and depends on its composition and structure, making its accurate determination a crucial method for protein identification. Furthermore, the pI serves as a critical metric in various biochemical and proteomic analyses, including two-dimensional gel electrophoresis (2D-PAGE), X-ray crystallography, and liquid chromatography-mass spectrometry (LC-MS).
Based on capillary isoelectric focusing (cIEF), MtoZ Biolabs offers precise determination of the pI of any protein. Compared to traditional slab gel methods, cIEF boasts advantages such as short runtime, high precision, and minimal sample consumption. This technology is applicable to a variety of amino acids, peptides, recombinant proteins, enzymes, monoclonal antibodies, and has been widely utilized in clinical analysis.
Sample Submission Requirements
Protein sample concentration > 0.5 mg/mL, purity > 90%, minimum quantity not less than 50 pmol.
Deliverables
In the technical report, MtoZ Biolabs will provide you with a detailed report, including:
1. Experimental Procedures
2. Relevant Data
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