Protein Mass Fingerprinting Service

Protein mass fingerprinting is a protein identification technique based on mass spectrometry. It generates a distinct "molecular mass profile" by analyzing peptides produced from protein digestion. This method enables rapid and precise identification of proteins by comparing the profile against protein databases. It is widely applied in proteomics research, biopharmaceutical development, and quality control processes.

 

In protein studies, molecular mass analysis is crucial for understanding protein structure and function. The mass fingerprinting technique utilizes the molecular mass data of peptides to identify and characterize proteins. MtoZ Biolabs, with its advanced mass spectrometry platform and a team of experienced experts, provides high-quality Protein Mass Fingerprinting Service to support your research and development needs.

 

Services at MtoZ Biolabs

Our Protein Mass Fingerprinting Service provides the following:

1. Protein Identification: After enzymatic digestion of protein samples, a mixture of specific peptides is generated. The molecular masses of these peptides are measured by mass spectrometry, and the generated profile is compared with database entries to accurately identify the protein.

2. Protein Modification Analysis: This includes the detection of amino acid substitutions, truncations, and other modifications, and evaluates their impact on protein molecular mass, helping to reveal functional changes in the protein.

3. Post-translational Modifications (PTMs) Analysis: Using mass spectrometry profiling, we identify post-translational modifications (such as phosphorylation, methylation, acetylation) that help elucidate the regulatory mechanisms of proteins.

4. Quality Control & Consistency Monitoring: In biopharmaceutical production, mass spectrometry profiling is used to monitor product purity and consistency, ensuring stable quality across production batches.

 

Analysis Workflow

1. Sample Separation: Initial separation of protein samples via 1D or 2D PAGE.

2. Protein Digestion: Target bands or spots are excised and digested to yield peptides.

3. Mass Spectrometry Analysis: Peptide mass/charge ratios are analyzed using mass spectrometers (e.g., MALDI-TOF or LC-MS/MS).

4. Data Comparison: Experimental mass spectrometry data are matched against theoretical peptide masses from protein databases for protein identification.

5. Reporting: We provide detailed reports with protein identification and in-depth analysis results.

 



 

Service Advantages

1. Advance Analysis Platform: MtoZ Biolabs established an advanced protein analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein analysis data providing clients with a comprehensive data report.

4. Experienced Technical Team: Our experts offer professional consultation and technical support tailored to your research needs.

5. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.

 

Applications

• Proteomics Research: Large-scale identification and analysis of proteins in biological systems.
• Biopharmaceutical Development: Structural identification of candidate drug proteins, detection of modifications and mutations, ensuring drug quality.
• Disease Biomarker Discovery: Identification of biomarkers associated with diseases by analyzing differential protein expression.
• Quality Control & Regulatory Compliance: Monitoring product consistency and purity in biopharmaceutical production to meet regulatory standards.

 

Case Study

1. Protein Mass Fingerprinting of Hemp Seeds



Cattaneo, C. et al. Plants. 2023.

 

 

FAQ

Q1: Is this service suitable for all types of protein samples?

Yes, Protein Mass Fingerprinting is a versatile technique, but different protein samples may require specific preparation or optimization. Special protein types (e.g., membrane proteins, glycoproteins, or high-molecular-weight proteins) might need additional sample preparation (e.g., membrane protein extraction or glycosylation removal) to ensure effective digestion and mass spectrometry analysis. Sample purity and concentration also play critical roles in successful protein identification. MtoZ Biolabs' team will tailor the analysis approach to your sample type and research objectives, ensuring accurate and reliable results.

 

Deliverables

1. Comprehensive Experimental details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs offers additional services, including protein sequencing, post-translational modification analysis, and protein-protein interaction studies. These services complement protein mass fingerprinting, providing a deeper understanding of protein structure and function. For more information or to request customized solutions, please contact our technical team directly.