Quantitative Methylomics Service

Methylation represents one of the most prevalent post-translational modifications, chiefly affecting transcription factors and histones, though it also modifies a subset of cytoplasmic proteins. This modification includes monomethylation, symmetric/asymmetric dimethylation, and trimethylation on arginine (R) and lysine (K) residues. Arginine methylation plays critical roles in RNA processing, gene transcription, DNA damage repair, protein translocation, and signal transduction, whereas lysine methylation primarily influences histone function and epigenetic regulation of gene transcription. Targeted enrichment of methylated peptides using modification-specific antibodies, followed by LC-MS/MS analysis, facilitates extensive quantitative and qualitative assessment of protein methylation.

 

MtoZ Biolabs employs the Orbitrap Fusion Lumos mass spectrometer and nanoLC from Thermo Fisher for Quantitative Methylomics Service. Clients need only submit their experimental aims and samples; MtoZ Biolabs handles all subsequent steps, including protein extraction, digestion, methylation specific peptide enrichment, peptide separation, mass spectrometric analysis, analysis of raw data, and bioinformatics.

 

Technical Principles

Protein methylation typically occurs at arginine and lysine residues, where up to three methyl groups can be added. The molecular weights of mono-, di-, and trimethylation increase by 14.02Da, 28.03Da, and 42.05Da, respectively. Mass spectrometry can precisely measure these molecular weight changes and identify methylated peptides and modification sites.

 

Analysis Workflow

 



 

Experimental Instruments

1. NanoLC: Vanquish NEO

2. MS: Orbitrap Fusion Lumos With ETD

 

Sample Submission Requirements

 



Note：

1. If you are providing tissue samples, please ship them on dry ice to ensure their integrity during transport.

2. For protein samples, standard tissue or cell lysis buffers can be used for protein extraction.

3. Please use sufficient dry ice and choose an expedited shipping method to minimize the risk of sample degradation during transit.

4. Prior to conducting the formal experiments, we will assess the quality of your samples, and proceed with the analysis only after they meet our testing standards.

 

Service Advantages

1. High Throughput

Capability to identify and quantify thousands of proteins simultaneously.

 

2. Complete Coverage

Digestion with 2-3 enzymes guarantees comprehensive protein analysis.

 

3. High Sensitivity

Applicability to low-abundance methylated proteins

 

4. State-of-the-Art Equipment

The Orbitrap Fusion Lumos, noted for its superior resolution and sensitivity.

  

Deliverables

In the technical report, MtoZ Biolabs will provide you with detailed technical information, including: 

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Identified Methylation Sites

4. Mass Spectrometry Images

5. Raw Data

 

Our team, seasoned in protocol optimization, ensures top-quality results in protein methylation studies. For specific inquiries, please contact us directly.