Protein Monomethylation Analysis

Protein methylation, a common post-translational modification (PTM), plays a pivotal role in regulating biological processes such as gene expression, signal transduction, and chromatin organization. Based on the level of methyl group addition, protein methylation occurs in three forms: monomethylation, dimethylation, and trimethylation. Monomethylation, specifically, involves the addition of one methyl group to lysine (Lys) or arginine (Arg) residues, facilitating precise regulation of processes such as gene control, chromatin state modulation, and signal transduction. Compared to dimethylation and trimethylation, monomethylation exhibits greater versatility in modulating various cellular functions.

 

 

Figure 1. Lysine Monomethylation

 

MtoZ Biolabs offers comprehensive Protein Monomethylation Analysis services utilizing high-performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced technique integrates the separation capabilities of HPLC with the high sensitivity and specificity of MS, enabling accurate identification and quantification of monomethylation sites. Our service assists researchers in gaining a deeper understanding of monomethylation’s roles in cellular metabolism, gene regulation, and disease mechanisms. Covering the entire analytical workflow, from sample preparation to data interpretation, we ensure high-quality experimental results and actionable insights for our clients.

 

Analysis Workflow

 

Services at MtoZ Biolabs

1. Targeted Protein Monomethylation Analysis

MtoZ Biolabs' targeted protein monomethylation analysis service specializes in the precise detection and quantification of monomethylation modifications on specific proteins. Utilizing high-resolution mass spectrometry combined with enrichment techniques like immunoprecipitation (IP), we accurately identify monomethylation sites, enabling researchers to investigate the modification’s role in gene expression regulation, cell cycle control, and disease progression. This service is ideal for detailed functional studies of individual proteins, particularly in the fields of cancer, metabolic disorders, and signal transduction research

 

2. Monomethylation Proteomics

Our monomethylation proteomics service offers a comprehensive analysis of monomethylation across the entire proteome. Leveraging a high-throughput mass spectrometry platform, we deliver a panoramic view of methylation modifications across various samples, uncovering extensive methylation patterns. This service is extensively used in epigenetic studies, cancer research, and metabolomics, aiding researchers in identifying potential biomarkers and regulatory networks, ultimately providing a complete protein modification map to better understand disease mechanisms.

 

Service Advantages

1. Advance Analysis Platform

MtoZ Biolabs established an advanced protein PTM analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

2. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate PTM proteomics analysis data providing clients with a comprehensive data report.

 

3. Comprehensive Coverage

Using both TMT/iTRAQ labeling and Label-Free techniques for sample analysis significantly enhances experimental efficiency while ensuring high-quality data.

 

4. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Applications

1. Disease Mechanism Research: Investigating monomethylation’s role in complex diseases such as cancer and neurodegenerative disorders, helping to identify biomarkers and potential therapeutic targets.

2. Gene Expression Regulation: Exploring the influence of protein monomethylation on chromatin architecture and gene expression activity.

3. Signal Transduction: Elucidating the regulatory effects of monomethylation in cellular signaling pathways, shedding light on key molecular communication processes.

4. Drug Development: Supporting drug discovery and optimization by targeting specific monomethylation modifications.

 

Application Case

Characterization of Lysine Monomethylome and Methyltransferase in Model Cyanobacterium Synechocystis sp. PCC 6803



 

Lin, X. H. et al. Genomics Proteomics Bioinformatics, 2020.

 

With our cutting-edge mass spectrometry platform and extensive expertise, MtoZ Biolabs offers not only precise monomethylation analysis but also services for a range of methylation modifications (monomethylation, dimethylation, trimethylation) and other PTMs, including propionylation, acetylation, and phosphorylation. We are committed to providing comprehensive technical support to advance your research. Please contact us for further information.