Protein Phosphorylation Modification 4D-DIA Label-Free Quantification Technique
Protein phosphorylation is a pivotal form of post-translational modification, essential for cellular signaling, cell cycle regulation, growth, differentiation, and apoptosis. Investigating protein phosphorylation is thus of immense scientific and clinical importance. Recently, the advent of 4D-DIA label-free quantification technology has introduced a novel approach to proteomics analysis. This method integrates ion mobility chromatography (IMC), tandem mass spectrometry (MS/MS), and data-independent acquisition (DIA) to enhance the quantification of protein phosphorylation.
Principle of Operation
4D-DIA technology initially employs ion mobility chromatography (IMC) for the separation of mixed samples. Subsequently, tandem mass spectrometry (MS/MS) is utilized for detailed analysis. Ultimately, data-independent acquisition (DIA) is used for comprehensive data collection and quantification.
Key Advantages
1. Enhanced Quantification Accuracy
The implementation of data-independent acquisition minimizes potential biases associated with labeling, thus enhancing quantification accuracy.
2. Extended Quantification Range
4D-DIA facilitates full-range quantification from low to high abundance, surpassing the limitations inherent in traditional labeling techniques.
3. Reduced Sample Requirement
The use of ion mobility chromatography allows for efficient protein separation and quantification even in low-concentration samples, thereby significantly reducing the amount of sample needed.
4D-DIA label-free quantification technology offers an efficient and precise method for protein phosphorylation quantification. This advancement is significant for comprehensively understanding the role of protein phosphorylation in biological processes and identifying novel therapeutic targets.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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