Protein Profiling Service

Protein profiling services systematically identify and characterize proteins in samples, providing comprehensive insights into protein types, quantities, modifications, interactions, and functions. Protein profiling enables in-depth analysis of cellular processes, disease mechanisms, and signaling pathways, offering essential support for biomarker discovery, drug target screening, and protein function studies.

 

Proteins serve as the primary functional components of cells. Investigating proteins allows for a deeper understanding of biological processes, disease onset, and drug mechanisms. The objective of protein profiling service is to identify as many proteins as possible within a sample. This is achieved by lysing, digesting, and separating protein samples, followed by detecting and identifying the separated peptide segments using high-resolution mass spectrometry. This process enables the determination of the proteins present in the sample. Protein profiling service permits the identification of all proteins within a sample, allowing for the simultaneous identification of hundreds to thousands of proteins.

 

Service at MtoZ Biolabs

MtoZ Biolabs offers protein profiling services based on mass spectrometry-based proteomics technology, combined with efficient protein digestion and peptide separation strategies. Utilizing primary mass spectrometry (MS1) and secondary mass spectrometry (MS2), we achieve precise peptide detection and high-coverage protein identification through database matching. With high-throughput and high-sensitivity analytical capabilities, our services are widely applied in precision medicine, drug development, and fundamental biological research, providing researchers with comprehensive and accurate proteomics solutions. If you are interested in our services, please feel free to contact us.

 

Service Advantages

1. High-Coverage Protein Identification Capability

MtoZ Biolabs utilizes advanced mass spectrometry platforms and optimized analytical workflows to comprehensively identify a large number of proteins in samples, including low-abundance proteins, providing high-coverage proteomics data for research.

 

2. Dynamic Analysis of Diverse Samples

Our service supports the analysis of various biological samples, including tissues, cells, and biofluids, enabling dynamic monitoring of protein changes under different conditions or time points, thereby supporting the study of complex biological mechanisms.

 

3. Efficient Data Processing and Professional Interpretation 

Backed by an experienced bioinformatics team and optimized database search algorithms, we can rapidly process large-scale data and provide professional result interpretation, helping researchers uncover deeper biological insights.

 

4. Fast Turnaround Time  

MtoZ Biolabs has optimized its mass spectrometry workflows and sample processing techniques to significantly shorten the turnaround time for protein profiling services. Leveraging advanced equipment and a professional team, the process from sample submission to data delivery typically takes only a few weeks, ensuring clients receive high-quality data promptly and greatly enhancing research efficiency.

 

Case Study

1. Serum Protein Profiling Reveals a Landscape of Inflammation and Immune Signaling in Early-stage COVID-19 Infection

 



Hou, X. et al. Mol Cell Proteomics. 2020.

 

2. Longitudinal Plasma Protein Profiling using Targeted  Proteomics and Recombinant Protein Standards

 



Kotol, D. et al. J Proteome Res. 2020.

 

Applications

Protein profiling services can be used in:

1. Identifying Drug Target

 



Ha, J. et al. Cell Chem Biol. 2021.

 

2. Discovering Disease Biomarker

 



Peter, Pichler. et al. bioRxiv. 2023.

 

FAQ

1. How to Effectively Address the Complexity of Biological Samples?

The complexity of biological samples can be addressed by optimizing enrichment and separation techniques. Methods such as affinity chromatography, IMAC, or multi-dimensional liquid chromatography effectively enhance the detection sensitivity of low-abundance or specifically modified proteins. Additionally, removing high-abundance proteins like albumin and utilizing high-resolution mass spectrometers with sensitive analytical capabilities significantly improve the detection efficiency of low-abundance proteins, ensuring comprehensive capture of critical proteins in complex samples.

 

2. How to Solve the Challenge of Wide Dynamic Range in Proteins?

The challenge of wide dynamic range in proteins can be solved by employing high-sensitivity, wide-dynamic-range mass spectrometers such as the Orbitrap series, along with separation and fractionation techniques like SCX or RP-HPLC to narrow the analytical scope. Moreover, using quantitative labeling methods (e.g., TMT) combined with optimized mass spectrometry data acquisition strategies ensures precise detection of low-abundance proteins without losing high-abundance protein information, enabling comprehensive and accurate protein profiling analysis.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Protein Profiling 

5. Mass Spectrometry Image

6. Raw Data

 

If you are interested in our protein profiling services, please contact us freely.