Proteins Analysis Service

Protein analysis is a fundamental step in studying biological systems, and it is essential in various fields such as drug development, disease diagnosis, and agricultural breeding. At MtoZ Biolabs, we provide comprehensive proteins analysis service using advanced mass spectrometry techniques combined with our expertise in proteomics. With the aim of advancing your research and maximizing your success, our proteins analysis service is designed to be reliable, efficient, and precise.

 

Services at MtoZ Biolabs

Our proteins analysis service is based on state-of-the-art mass spectrometry, including LC-MS/MS and MALDI-TOF/TOF. We utilize these technologies to identify and quantify proteins in biological samples. The proteins are first separated by HPLC and then ionized before being introduced into the mass spectrometer. The mass-to-charge ratios of the ions are measured to determine the molecular weight of the proteins. By comparing the obtained mass spectra to a database of known protein sequences, we can identify the proteins present in samples.

 

Analysis Workflow

 

 

 

Service Advantages

 



 

 Case Study

1. Drug Target Identification by Chemical Proteomics

The authors conducted target screening experiments using a chemical probe, identifying 59 proteins consistently across three biological replicates as potential targets. KEGG enrichment analysis and GO analysis provided insights into the evaluation of these targets, highlighting Immunity-related GTPase family M protein 1 (IRGM1) as a critical regulator of innate immunity and inflammatory responses, which was identified as a promising cellular target. Proteins analysis service offers comprehensive solutions for target screening, functional enrichment analysis, and the identification of key regulatory proteins for biomedical research.

 



Li T, et al. Nat Commun. 2024.

Figure 1. Target Identification by Chemical Proteomics

 

2. Protein Analysis of Colorectal Cancer Patient

This study applies a workflow for high-resolution spatial proteomics analysis of colorectal cancer (CRC) FFPE slides using DIA-MS (data-independent acquisition mass spectrometry). Through tissue section expansion, hydrogel dotting, protein digestion, and mass spectrometry analysis, the method effectively captures proteomic information from nanoscale samples (6,751 proteins and 58,866 peptides). The expanded sections exhibit high precision in spatial resolution and protein abundance detection. The results reveal significant differences in protein expression across different regions (normal, adjacent, and tumor tissues), with regional-specific proteomic features highlighted through clustering analysis. This approach provides an innovative and efficient strategy for spatial proteomics research on FFPE samples. Proteins analysis service offers robust solutions for investigating spatial proteomics in complex biological samples. Combining advanced mass spectrometry techniques, it enables precise detection and quantification of proteins across distinct tissue regions.

 



Dong Z, et al. Nat Commun. 2024.

Figure 2. Proteomic Analysis of Colorectal Cancer Tissue Sections Based on DIA-MS

 

Applications

Our proteins analysis service can be applied in various fields, including but not limited to:

 

· Biomedical research: Identifying and quantifying proteins involved in diseases can help understand the disease mechanism and develop potential therapeutic targets.

· Drug development: Our proteins analysis service can assist in the discovery and validation of drug targets, as well as in the evaluation of drug efficacy.

· Agricultural breeding: Proteins analysis service in MtoZ Biolabs can help to understand the genetic traits of crops and livestock, promoting the breeding of high-quality varieties.

 

Sample Submission Suggestions

For successful protein analysis, please ensure your samples meet our submission requirements:

 

· Samples should be clear without visible impurities.

· The protein concentration should be higher than 1 mg/mL.

· Ensure your samples are stored and shipped under appropriate conditions to prevent protein degradation.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Protein Analysis 

5. Mass Spectrometry Image

6. Raw Data

 

At MtoZ Biolabs, we are committed to providing our clients with the most reliable and highest quality proteins analysis service. Please feel free to contact us if you have any questions about our service.