Protein Sequencing

The primary structure of a protein is composed of a linear sequence of amino acids and serves as the foundation for the protein's structure. MtoZ Biolabs provides high-precision protein sequence analysis services to determine the complete amino acid sequence of proteins. This information is crucial for protein identification, the design of molecular cloning probes, and the synthesis of immunogenic peptides.

 

Services at MtoZ Biolabs

MtoZ Biolabs employs two main techniques for protein sequence analysis: Edman degradation and mass spectrometry (MS) analysis. Our services encompass mass spectrometry analysis, including complete sequence analysis and amino acid composition analysis, as well as N-terminal sequence analysis using Edman degradation.

 

1. Mass Spectrometry Analysis

We use high-resolution mass spectrometry to ensure comprehensive coverage of the target protein's sequence. Mass spectrometry offers higher sensitivity and speed compared to Edman degradation and is effective in identifying proteins that are terminally blocked or chemically modified. Additionally, our Thermo Scientific™ Orbitrap Fusion™ Lumos™ mass spectrometer, equipped with HCD and ETD dual fragmentation technologies, enhances the detection sensitivity for low-abundance peptides and the accuracy of peptide identification. This technology supports detailed analysis of the protein's N-terminal, C-terminal, and full sequence.

 



Figure 1. Mass Spectrometry Sequencing Diagram

 

2. Edman Degradation

Edman degradation is a classical chemical sequencing method suitable for unmodified N-terminal sequence analysis. Although it has limitations, such as being unable to analyze proteins with blocked N-termini or chemical modifications, it remains an important means of obtaining N-terminal sequence information. MtoZ Biolabs uses advanced Edman degradation technology capable of accurately analyzing up to 30 amino acids of the N-terminal sequence.

 



Figure 2. Edman Degradation Sequencing Diagram

 

Sample Submission Requirements

Sample preparation is a critical prerequisite for protein sequence analysis. We recommend using methods such as SDS-PAGE, HPLC, or capillary electrophoresis, choosing the most suitable preparation method based on the sample's purity, quality, and experimental needs. For clients unable to prepare samples themselves, MtoZ Biolabs offers comprehensive sample preparation services.

 

We accept various forms of samples, including PVDF membranes, gel spots, and solutions. The method of shipping will be chosen based on the sample's form and stability, such as ice packs or dry ice, to ensure the samples maintain their integrity and activity before analysis.

 

MtoZ Biolabs is committed to providing a one-stop protein sequencing solution for researchers and biotechnology companies. Feel free to contact us by phone or email, and we will provide professional consultation and support.