Proteomic Analysis of TMT Technology

As the field of proteomics advances, qualitative analysis of protein types and modifications alone is no longer sufficient to meet the demands of scientific research. Under these circumstances, quantitative proteomics technologies emerged and have become one of the hot topics in life sciences research in recent years. The quantitative technology in proteomics quantifies the expression and abundance of proteins based on the known protein types and the signal strength given by mass spectrometry technology.

 

Tandem Mass Tag (TMT) is a relative and absolute quantitative proteomics technology developed by Thermo Fisher Scientific based on in vitro isotope labeling. The basic process uses isotopes to react with the amino group of peptide segments. By using the principle of equal weight labeling, different samples are labeled, and the ion signal response intensity of the same peptide segment in different samples is detected on the machine. Through the analysis of the mass spectrometer, proteins in multiple samples (up to 18 tags) can be quantified and identified simultaneously.

 

The Advantages of TMT Technology Compared With Traditional Single-Tag Technology

1. Multiple Labeling and High Throughput

TMT technology can compare multiple samples at the same time, significantly increasing experimental efficiency, greatly shortening the experimental cycle, and reducing experimental costs.

 

2. High Sensitivity and Accuracy

TMT technology performs excellently in terms of quantification accuracy and dynamic range. It can accurately measure changes in protein abundance in different samples, providing more precise data support for biological research.

 

3. Strong Universality

TMT technology is suitable for analyzing a variety of complex samples, including cells, tissues and even body fluid samples, providing more possibilities for disease diagnosis and drug development.

 

Due to its high throughput, high sensitivity, and broad applicability, TMT technology has become a commonly used quantitative proteomics method. In recent years, this technology has been widely used in scientific research, providing researchers with a powerful tool to deeply explore protein function and regulation mechanisms. MtoZ Biolabs uses Thermo Fisher's Q ExactiveHF mass spectrometry platform, Orbitrap Fusion mass spectrometry platform, and Orbitrap Fusion Lumos mass spectrometry platform combined with Nano-LC to provide TMT-based quantitative proteomics analysis services. In addition, there is the in vivo labeling SILAC technology, which is suitable for different samples and scenarios, and can meet your different needs.