Proteomics Technology 4D

In 2018, 4D proteomics officially entered the public view. Compared to traditional 3D proteomics, 4D proteomics adds the concept of ion mobility, in addition to retaining retention time, m/z, and intensity, thereby significantly improving scanning speed and detection sensitivity.

 

Ion mobility, also known as ion drift rate, refers to the speed at which an ion passes through a certain gaseous area under unit electric field strength, which is often related to the shape, size, and charge state of the ion. By introducing ion mobility, 4D proteomics can better distinguish peptides with similar m/z, thereby improving the accuracy and reliability of protein quantification.

 

The principle of 4D proteomics is based on the timsTOF Pro mass spectrometer, combined with PASEF (Parallel Accumulation Serial Fragmentation) and TIMS (Trapped Ion Mobility Spectrometry), which can repetitively measure all detected ions' collision cross sections (CCS). According to the different CCS of peptide ions when they collide with the buffer gas in the drift tube, ions can be separated by size and shape.

 

Service Advantages

1. More Accurate Qualitative Analysis

Ion mobility separation can effectively distinguish between modifications and isomers, making modification identification results more accurate.

 

2. Identify More Proteins

The throughput of 4D proteomics can be increased to 10 times that of ordinary proteomics, effectively reducing the experimental cycle.

 

3. More Accurate Quantification

The instrument is more stable, and the correlation coefficient between 1-96 pin samples can reach 0.95 or above.

 

4. Higher Sensitivity

The sample volume of traditional proteomics is at the μg level, while the minimum protein amount for 4D proteomics can be as low as 200ng, which is 1/10 of the sample volume of ordinary proteomics, suitable for various trace samples.

 

Currently, 4D proteomics has shown broad development prospects and strong vitality in various fields such as basic research, translational medicine, and clinical proteomics. MtoZ Biolabs provides 4D proteomics research services based on timsTOF Pro, assisting with micro-sample proteomics, large sample group medicine, and high-throughput modification proteomics research. Inquiries are welcome!