Purine Metabolism Service
- Disease Research
- Drug Discovery
- Biological Research
- Type of samples: Plasma, serum, cell, tissue, etc.
- Quantity: At least 500 μl liquid, 1 x 10⁷ cells, or 500 mg tissue.
- Storage and Shipping: Samples should be stored at -80℃ and shipped with dry ice.
- Note: Avoid repeated cycles of freezing and thawing
Purines are essential biomolecules that play critical roles in various cellular processes, including DNA and RNA synthesis, energy metabolism, and signal transduction. The purine metabolism pathway is a complex network of enzymatic reactions responsible for the synthesis, interconversion, and degradation of purine nucleotides. This pathway is vital for maintaining cellular homeostasis and function.
In biological systems, purine metabolism begins with the synthesis of inosine monophosphate (IMP) via the de novo pathway or through salvage pathways that recycle purines from degraded nucleotides. IMP serves as a precursor for the synthesis of adenosine monophosphate (AMP) and guanosine monophosphate (GMP), which are further converted into their respective triphosphates, ATP and GTP. These nucleotides are fundamental for energy transfer, cellular signaling, and as building blocks for nucleic acids.
Key enzymes involved in purine metabolism include glutamine-PRPP amidotransferase, adenylosuccinate synthetase, adenylosuccinate lyase, and xanthine oxidase. Metabolites such as hypoxanthine, xanthine, and uric acid are crucial intermediates and end-products of purine catabolism. Dysregulation of purine metabolism is associated with various diseases, including gout, hyperuricemia, and certain cancers, making it a critical area of biomedical research.
Figure 1. The Pathway of Purine Metabolism
At MtoZ Biolabs, we understand the significance of purine metabolism and offer a comprehensive purine metabolism service. Our purine metabolism service is based on state-of-the-art high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) technology. This tool allows us to separate, identify, and quantify each purine metabolite with high accuracy and sensitivity. Specifically, we carry out an unbiased profiling to detect all purine metabolites, followed by a targeted metabolomics analysis to quantitatively analyze specific metabolites of interest. This approach ensures a comprehensive understanding of the purine metabolic pathway.
Analysis Workflow
Service Advantages
1. Expertise
A team of highly skilled scientists with rich experience in purine metabolism studies ensures the delivery of exceptional results.
2. Advanced Techniques
Advanced HPLC-MS/MS technology provides accurate and sensitive detection.
3. Fast Turnaround
The process from sample handling to report generation is efficient, shortening the analysis cycle.
4. Comprehensive Analysis
The report covers both unbiased profiling and targeted metabolomics analysis.
Applications
Sample Submission Requirements
For more details, please feel free to get in touch with our customer service team.
Deliverables
1. Experimental Procedures
2. Relevant High-Performance Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Purine Metabolism Analysis
4. Raw Data
5. Custom Analysis Report
With the purine metabolism service offered by MtoZ Biolabs, you can expect reliable results to accelerate your scientific research. Contact us today to learn more about our services.
How to order?
