Purity Analysis of Antibody Drugs (CE-SDS, SDS-PAGE, SEC, RP, etc.)

Antibody drugs are a class of drugs that treat diseases through artificially synthesized antibodies. They achieve therapeutic purposes by specifically binding to target molecules. Common types of antibody drugs include monoclonal antibodies, artificially synthesized antibody fragments, immunotoxins, antibody-drug conjugates, etc. Antibody drugs have shown significant therapeutic effects in the treatment of various diseases, such as cancer, autoimmune diseases, inflammatory diseases, immune regulation, and ophthalmic diseases. In antibody drug research, dissecting the higher structure is very important for understanding its pharmacological activity, stability, transmission route, and toxicity. The purity of antibody drugs is key to protein crystallization and rigorous structural and functional research. Impure samples can lead to inaccurate results of the entire experiment. In addition, protein purity is a very important feature for further protein research and biopharmaceutical applications.

 

The antibody drug purity analysis method using capillary electrophoresis (CE-SDS) or SDS-PAGE electrophoresis is simple and has a very low cost, and it is very sensitive in determining the protein components in the sample, so it has become one of the commonly used techniques for protein purity analysis. SDS-PAGE separates proteins based on the different migration rates produced by the differences in charges carried by different proteins and different molecular sizes.

 

Molecular sieve HPLC, also known as size exclusion chromatography (SEC), Small molecular weight compounds enter the gel pores and have a longer retention time; large molecular weight compounds cannot enter the pores and will be eluted earlier. The advantage of molecular sieve chromatography is that it can handle at least 1nmol of target protein and obtain a good separation effect. The separation time is also very short, generally within 3 hours, and the chromatographic separation peak obtained is also smaller.

 

The Advantages of SEC for Protein Purification

1. Performing Buffer Exchange and Desalting

2. Separating Similar Species that Are Difficult to Separate by Other Purification Technologies (Such as Protein Fragments and Oligomers)

3. Being Compatible with Various Solvents

4. Not Relying on Any Special Form of Protein for Retention and Elution

 

MtoZ Biolabs provides customers with drug quality research services that comply with global drug regulatory laws and regulations. Based on various technology platforms, we provide you with a series of services for the characterization of antibody drug purity, including CE-SDS, SDS-PAGE, SEC, RP, etc., which can be used for the separation and determination of various antibodies to meet different scientific research needs. We welcome free consultations and learn more details!