Pyruvic Acid and its Derivatives Assay

Pyruvic acid is a vital metabolite produced during glycolysis, central to energy metabolism. Under anaerobic conditions, glucose is converted into pyruvic acid, which is crucial for rapid energy production in low-oxygen environments. When oxygen is available, pyruvic acid is converted into acetyl-CoA by the pyruvate dehydrogenase complex, entering the tricarboxylic acid (TCA) cycle for complete oxidation and energy production. Pyruvic acid also serves as a precursor for synthesizing various biomolecules such as amino acids, fatty acids, and nucleotides.

 

The derivatives of pyruvic acid, including lactic acid, acetyl-CoA, oxaloacetate, and alanine, play significant roles in physiological processes. Lactic acid is produced from pyruvic acid under anaerobic conditions, mainly during intense exercise or hypoxia. Acetyl-CoA, produced via the pyruvate dehydrogenase complex, enters the TCA cycle to participate in energy metabolism. Oxaloacetate is essential for gluconeogenesis and fatty acid synthesis. Alanine, generated from pyruvic acid through transamination, is a crucial substrate for protein synthesis.

 

Pyruvic acid and its derivatives are key players in energy production, signal transduction, and metabolic regulation. Abnormal levels of these metabolites are linked to various metabolic diseases and pathological conditions, such as diabetes, cardiovascular diseases, and cancer. Therefore, accurate quantitative and qualitative analysis of pyruvic acid and its derivatives is critical in basic research, clinical diagnostics, and drug development.

 

 

Figure 1. Molecular Structure of Pyruvic Acid

 

MtoZ Biolabs offers Pyruvic Acid and its Derivatives Assay Service using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). This advanced method combines the separation capabilities of UPLC with the detection power of MS, ensuring high sensitivity and specificity in pyruvic acid and its derivatives detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity and Precision

UPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of pyruvic acid and its derivatives.

 

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

 

3. Wide Applicability

Suitable for various biological samples, including blood, urine, and tissues.

 

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

 

Sample Submission Requirements

Make sure the sample volume is greater than 0.2 g or 0.2 mL. For detailed sample submission information, please contact our customer service.

 

Applications

1. Basic Research: Study the role of pyruvic acid in cellular metabolism and energy production.

 

2. Clinical Diagnostics: Monitor pyruvic acid levels to assist in diagnosing diabetes, cardiovascular diseases, and metabolic disorders.

 

3. Drug Development: Evaluate the impact of drugs on pyruvic acid metabolic pathways and identify potential therapeutic targets.

 

4. Food Science: Detect pyruvic acid content in food to ensure safety and nutritional value.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Pyruvic Acid and its Derivatives

4. Raw Data

5. Custom Analysis Report