Quantitative Proteomic Study of Human Cell Phosphorylation

In modern biomedical research, protein phosphorylation is an important post-translational modification method, which plays a crucial role in regulating cell signal transduction, cell growth, differentiation, and death. Human cell phosphorylation quantitative proteomics research aims to fully understand the role of protein phosphorylation in cellular physiological and pathological processes, providing a basis for disease diagnosis, treatment, and new drug development.

 

With the development of mass spectrometry technology, we can now accurately quantify the phosphorylation sites at the whole proteome level, revealing the specific role of phosphorylation in cellular physiological and pathological processes. MtoZ Biolabs, based on Thermo Fisher's Q ExactiveHF mass spectrometry platform, Orbitrap Fusion mass spectrometry platform, Orbitrap Fusion Lumos mass spectrometry platform combined with Nano-LC, launched a service package for phosphorylation quantitative proteomics analysis, which can accurately identify and quantify the phosphorylation sites in cells and their dynamic changes. This technique can carry out high-throughput phosphorylation analysis at the whole proteome level, revealing the wide-ranging impact of phosphorylation events within cells.

 

Service Advantages

1. High Sensitivity Detection

Advanced mass spectrometry technology can detect low abundance phosphorylated proteins and sites, ensuring the comprehensiveness and accuracy of the analysis.

 

2. Large-Scale Phosphorylation Site Analysis

Taking advantage of the high-throughput characteristics of mass spectrometry technology, thousands of phosphorylation sites can be analyzed simultaneously in one experiment, significantly increasing the coverage of the data and the efficiency of the research.

 

3. Quantitative Analysis and Dynamic Monitoring

By using labeled or unlabeled quantitative methods, the changes in phosphorylation levels can be accurately quantified, which can be used to monitor changes in cell status or the effects of drug interventions.

 

Applications

1. Cell Signal Transduction Research

Through phosphorylation proteomics analysis, a deeper understanding of the regulatory mechanism of cell signal transduction pathways can be gained, providing important clues for revealing cell function.

 

2. Disease Mechanism and Biomarker Discovery

Research on the changes in cell phosphorylation patterns under disease conditions can identify phosphorylation sites related to specific diseases, providing biomarkers for disease diagnosis and prognosis assessment.

 

3. Drug Action Mechanism Analysis

The impact of drugs on the phosphorylation status of proteins can be assessed to reveal the mechanism of action of the drug and its potential side effects.

 

MtoZ Biolabs' human cell phosphorylation quantitative proteomics research not only promotes the understanding of basic issues in cell biology but also provides new strategies and targets for disease treatment. Through precise phosphorylated protein analysis, we can gain a deeper insight into the complex biological processes within cells, laying a solid foundation for future biomedicine research and clinical applications.