Resources

Proteomics Databases



Metabolomics Databases

• • Analyze the Peak Patterns to Determine the Peptide Sequences

  The analysis of peptide mass spectra is a critical technique in bioinformatics, primarily utilized to determine the amino acid sequences of peptides. This process involves comparing the m/z values (mass-to-charge ratios) observed in the mass spectra with the calculated masses of theoretical amino acid residues to identify the peptide's sequence. The fundamental steps for analyzing peptide mass spectra are outlined below. 1. Obtaining the Mass Spectrum To begin with, the mass spectrum of the peptide needs...

• • Host Protein Residue Detection Process

  Quantification of host cell protein (HCP) residues is a standard quality control procedure in biopharmaceutical manufacturing processes. Its objective is to confirm that the residual levels of host cell proteins generated during production meet applicable regulatory requirements and product quality standards. The following provides an overview of a standard methodology for the detection of HCP residues:

• • Protein Profiling Methods

  Protein profiling methods encompass a suite of experimental techniques and tools designed to investigate the structure, function, expression levels, and interactions of proteins. As key players in biological processes, proteins perform diverse functions within organisms, including catalyzing biochemical reactions, mediating signal transduction, and supporting immune defense. To gain a comprehensive understanding of protein biology and their roles in health and disease, scientists have developed a rang......

• • How to Measure the Extinction Coefficient of a Fluorescent Protein

  The extinction coefficient of fluorescent proteins (FPs) is determined using a spectrophotometer, which measures their concentration by analyzing absorbance at specific wavelengths. The general procedure involves three steps: calculating the theoretical molar extinction coefficient, preparing the sample, and applying the Beer-Lambert law.

• • Serum Type IV Collagen Assay

  Serum type IV collagen is a distinct collagen located primarily within the basement membranes of the human body, serving as a vital component of the extracellular matrix. Its measurement is principally applied in the evaluation of renal diseases such as chronic kidney disease and diabetic nephropathy, as well as other conditions associated with basement membrane impairment, including liver cirrhosis and heart failure.

• • The Range of Molecular Weight Measured by MALDI-TOF

  Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) is a sophisticated analytical technique employed for determining ion masses. This method is capable of measuring a wide array of molecular masses, ranging from small molecules and oligonucleotides to peptides, proteins, and even large polymers and complexes. Molecular Mass Range: MALDI-TOF MS surpasses other mass spectrometry techniques in its ability to analyze an extensive range of molecular masses. Ideally, it....

• • HCP Coverage Test

  Host cell proteins (HCPs) refer to proteins that are unintentionally expressed by host cells used in the production of target proteins during biopharmaceutical manufacturing. These contaminating proteins may compromise the safety or efficacy of the drug, and therefore, their levels must be monitored and controlled through rigorous testing. Importance of HCP Coverage Testing HCP coverage testing evaluates the ability of antibody-based methods to efficiently detect various HCPs. The results from this test....

• • ICP-MS Determination of Mercury

  ICP-MS (Inductively Coupled Plasma Mass Spectrometry) is a highly versatile analytical technique used to quantify elemental concentrations and isotopic ratios with exceptional sensitivity and precision. Its capability to detect a wide range of elements with high accuracy has made ICP-MS an indispensable tool in fields such as environmental science, geology, biology, and materials science. Outlined below are the steps involved in the quantification of mercury using ICP-MS:

• • Type IV Collagen Test

  Significance of Type IV Collagen Detection: Type IV collagen is a crucial component of the basement membrane, serving as a primary structural element in numerous organs and tissues. It plays an essential role in various physiological processes such as cell migration, growth, differentiation, apoptosis, and disease progression. Consequently, Type IV collagen detection is extensively utilized in clinical diagnostics and disease research.

• • Bradford Protein Content Determination Experiment Report

  Objective: The aim of this experiment is to quantify protein concentration via the Bradford assay. Principle: The Bradford assay is based on the binding of Coomassie Brilliant Blue G-250 to proteins, forming a stable blue complex. The absorbance of this complex peaks at 595 nm when in an acidic environment, enabling the determination of protein concentration.