Resources

• • Mechanism of Protein Mutation Analysis

  Proteins are essential molecules in biological systems, participating in nearly all cellular processes. Mutations, which are changes in DNA sequences, can alter the amino acid sequences of proteins, thus impacting their structure and function. Analyzing protein mutations is a fundamental aspect of biological research, involving understanding their generation, effects, and detection methods.

• • Application of Protein Mutation Analysis

  Proteins are crucial molecules in living organisms that perform a wide array of functions, directly determining physiological states. Protein mutations, changes in the amino acid sequence of proteins, can impact protein function and subsequently affect biological processes. Hence, protein mutation analysis is pivotal in biological research, with extensive applications in disease research, drug development, and evolutionary biology.

• • Workflow of Protein Mutation Analysis

  Protein mutation analysis is a crucial method for investigating the function, structure, and interactions of proteins. By substituting or deleting specific amino acids in a protein sequence, researchers can determine the impact of these variations on the protein's properties and biological functions.

• • Mechanism of De Novo Peptide Sequencing

  Peptide sequencing is a critical technique for determining the order of amino acids in proteins or peptides. Unlike traditional methods that depend on known databases, De Novo peptide sequencing can independently deduce amino acid sequences directly through mass spectrometry techniques.

• • Principle of Protein Mutation Analysis

  Proteins are the primary agents of biological activities, with their functional diversity and complexity arising from their highly specific three-dimensional structures and amino acid sequences. However, amino acid residues in protein sequences can mutate, potentially impacting protein structure and function. Protein mutation analysis is crucial for understanding disease mechanisms, developing novel therapies, and advancing protein engineering techniques.

• • Application of De Novo Peptide Sequencing

  De Novo peptide sequencing is an emerging technology in biological and biomedical research. Unlike traditional peptide sequencing methods that rely on databases, De Novo peptide sequencing does not require any prior protein sequence information. This characteristic makes it particularly advantageous for studying unknown proteins and newly discovered organisms.

• • Workflow of De Novo Peptide Sequencing

  De Novo peptide sequencing is a technique used to determine the primary structure of proteins without relying on known sequences in a database. This method is particularly important for studying newly discovered proteins or organisms that have not been fully investigated. By analyzing mass spectrometry data, researchers can deduce the amino acid sequence of peptide fragments.

• • Advantages and Disadvantages of De Novo Peptide Sequencing

  De Novo peptide sequencing is a technique that allows for the determination of amino acid sequences in proteins or peptides without the need for a reference sequence. It holds a significant position in proteomics, particularly in analyzing proteins from unknown species, discovering new proteins, and sequencing antibodies. However, despite its remarkable advantages, this technology also faces some challenges and limitations in its application.

• • Principles of De Novo Peptide Sequencing

  De Novo peptide sequencing is a crucial biotechnological method extensively utilized in proteomics research. This technique deduces the amino acid sequence of peptides directly from mass spectrometry data without relying on existing protein or nucleic acid databases. It plays a vital role in studying newly discovered proteins, mutants, or protein expressions in non-model organisms.

• • N-Terminal Sequencing: Analysis of the Amino Acid Sequence Start

  N-terminal sequencing, specifically refers to the sequencing of the N-terminus of a protein, which is a method used to determine the starting portion of the amino acid sequence of a protein. The following is a detailed explanation of N-terminal sequencing: Figure 1. Protein N-terminal and C-terminal sequencing process   I. Principle: The classic method for N-terminal sequencing is Edman degradation.