Resources
Proteomics Databases
Metabolomics Databases
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• Protein Content Analysis Method
Protein content analysis methods are crucial for diverse fields, including food science, biotechnology, and pharmacology. These methods, including Kjeldahl nitrogen determination, colorimetric assays, UV absorption, HPLC, and mass spectrometry, each have distinct strengths and applications tailored to specific research needs. For instance, the Kjeldahl method remains a gold standard in food industries for measuring total protein content by analyzing nitrogen levels in complex samples. Meanwhile, col......
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• Protein Content Determination
Protein content determination refers to the precise measurement of protein concentration and content in a sample. Numerous methods are available for protein content determination, each with distinct advantages and limitations. The most commonly used methods include colorimetric assays, fluorescence techniques, mass-based methods (such as mass spectrometry), and immunological assays (e.g., ELISA). Accurate protein content determination plays a crucial role in evaluating the nutritional value of f......
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• LC-MS/MS Liquid Chromatography Mass Spectrometry
LC-MS/MS Liquid Chromatography Mass Spectrometry is a technology that integrates Liquid Chromatography (LC) with Tandem Mass Spectrometry (MS/MS). Liquid Chromatography is responsible for separating the components of a mixture, while Mass Spectrometry detects and identifies the molecular weight and structural information of these components. By combining these two techniques, LC-MS/MS achieves high sensitivity and specificity in complex analyses. This method has found widespread applications in variou......
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MS protein sequencing is a technology used for the identification and quantitative analysis of proteins. It determines the amino acid sequence of proteins by analyzing their mass spectrometry data, which provides insights into their structure and function. In disease research, this technology enables the identification of disease-relevant protein biomarkers and aids in uncovering the molecular mechanisms underlying various diseases. These capabilities hold promise for advancing early diagnosis and per......
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• MALDI-TOF MS Bacterial Identification
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Bacterial Identification (MALDI-TOF MS Bacterial Identification) is an advanced technology for microbial identification, offering rapid and accurate bacterial species identification. MALDI-TOF MS Bacterial Identification efficiently classifies and identifies bacteria by analyzing their protein spectra. Compared to traditional biochemical methods, MALDI-TOF MS significantly reduces time and resource consumption, typically prov......
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• Mass Spectrometry in Protein Sequencing
Mass spectrometry in protein sequencing is a technique used to analyze the amino acid sequence of proteins. This method offers detailed structural information about proteins by measuring the mass-to-charge ratio (m/z) of their fragments. With the advancement of genomics, scientists have realized that relying solely on genomic sequences is insufficient to fully understand the complex functions of organisms. Proteins, being the direct executors of cellular functions, are better understood through mass s......
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• Label-Free Quantification Proteomics
Label-free quantification proteomics is an advanced technique used to study protein expression levels and dynamic changes within biological systems. Unlike traditional labeling methods, this technique does not rely on isotopic or chemical labels, but instead directly measures the relative abundance of proteins in a sample using mass spectrometry. This method offers significant advantages for the relative quantification of proteins in complex biological samples, especially in situations involving limit......
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Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis (MALDI-TOF MS Analysis) is a powerful technique used to analyze macromolecules such as proteins, peptides, and polysaccharides. In proteomics, MALDI-TOF MS Analysis is employed to identify and characterize proteins in complex biological samples. By analyzing peptide mass fingerprints, the sequence and structure of proteins can be precisely determined, providing valuable information for disease research and drug devel......
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• Protein Quality Evaluation Methods
Protein quality evaluation methods are essential tools for assessing the nutritional value, bioavailability, and functional properties of proteins. These methods provide critical information for determining how well proteins meet dietary requirements and their potential for use in various food, pharmaceutical, and clinical applications. Current common protein quality evaluation methods include the Chemical Score, Amino Acid Score (AAS), Protein Digestibility-Corrected Amino Acid Score (PDCAAS), Protei......
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Impurity profiling involves identifying, quantifying, and managing non-target components present in samples across disciplines like chemistry, pharmaceuticals, and biotechnology. These impurities may include by-products, degradation products, residual solvents, or other foreign substances from synthesis processes. The objective of impurity profiling is to ensure the sample's purity and stability, thus enhancing the research outcomes' accuracy and reliability. This technique finds applications from bas......
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