Resources

Proteomics Databases



Metabolomics Databases

• • How Much Protein Is Needed for Edman Sequencing?

  In Edman protein sequencing, the amount of protein typically needed depends on various factors, such as the sensitivity of the sequencing equipment, the purity of the protein, and the complexity of the sequence. Traditionally, the Edman degradation method is used to determine the amino acid sequence of proteins or polypeptides, usually requiring about 1 to 100 picomoles (pmol) of protein sample.

• • How to Analyze Conserved Protein Sequences?

  The conserved protein sequences are sequences of proteins that remain unchanged or relatively stable throughout the evolution of species. These sequences usually play a crucial role in sustaining life activities. By analyzing conserved protein sequences, we can better understand their functions within organisms and their significance in species evolution. Below, we will introduce several commonly used methods for analyzing conserved protein sequences.

• • Mass Spectrometry Flow Protocol

  Materials and Equipment 1. Flow Mass Spectrometer 2. Samples 3. Flow Mass Spectrometry Reagent Kit 4. Polyurethane Filled Bottles 5. Sieve 6. Centrifuge 7. Pipette Tips 8. P1000 and P200 Pipettes 9. Melt Reagent Box 10. 1.5ml Centrifuge Tubes   Sample Processing 1. Melt the sample at room temperature. Transfer the sample to a new 1.5ml centrifuge tube. 2. Clean the sample with a cleansing solution. 3. Centrifuge the sample for 5 minutes at 10000rpm using a centrifuge. 4. Discard the supernatant.

• • What Does Protein Mass Spectrometry Generally Measure?

  Protein mass spectrometry detection is used to determine the mass, structure, and chemical modifications of proteins or polypeptides. Protein mass spectrometry detection is generally used for the following aspects:   1. Determination of Protein Mass By determining the mass of protein molecules, we can understand the relative molecular mass of proteins, which helps in protein identification.

• • Analysis of ADC Drug Biological Activity

  Antibody-drug conjugates (ADCs) are a new class of drugs that combine the advantages of both antibodies and small molecule toxins. Compared with traditional cytotoxins, ADCs have the advantages of strong targeting, less toxic side effects, etc. They can accurately target tumor cells, reduce side effects, and improve the therapeutic effect.   ADC drugs are mainly composed of monoclonal antibodies, linkers, and small molecule cytotoxins.

• • HCP Host Protein Residue

  Host cell proteins (HCP) refer to the protein components from the host cells of the production cell line in biological products, which mainly include the structural proteins and secretory proteins of the host cells. These proteins are diverse and their physical and chemical properties, such as isoelectric point, hydrophobicity, relative molecular weight, etc., often have significant differences.

• • Purity Analysis of Oligonucleotides

  Oligonucleotides are typically composed of short-chain nucleotides (deoxyribonucleotides or ribonucleotides) of less than 20, serving as short RNA and DNA oligomers. They can be used as probes to determine the structure of DNA or RNA, and are used in processes such as gene chips, electrophoresis, and fluorescence in situ hybridization.   Oligonucleotides are widely used in biology, agriculture, medicine, and other fields mainly through chemical synthesis.

• • Cell Gene Knockout Technology

  What is Gene Knockout Technology? Gene knockout (Gene Knockout) is a molecular biology technique that allows researchers to block or shut off the function of a specific gene to study its role and importance. This technique is achieved by introducing mutations into the gene, making it impossible for the gene to express properly or produce functional proteins.

• • Why Does Protein Sequencing Start at the N-Terminus?

  Degradation in protein sequencing usually starts from the N-terminus (amino terminal) mainly due to:   1. Enzyme Specificity The enzyme used for protein sequencing, such as phenyl isothiocyanate (PITC) in Edman degradation, specifically reacts with the N-terminal amino acid of the protein. This specificity ensures the accuracy and repeatability of the sequencing process.   2. Convenience of Sequencing Starting from the N-terminus, each amino acid can be removed step by step and identified.

• • Immunological Detection Method for Protein Acetylation Detection

  Acetylation is a common type of post-translational modification of proteins, which plays an important role in regulating the function and stability of proteins. Immunodetection is a commonly used method for detecting protein acetylation, including Western blot and Immunoprecipitation.