Resources

Proteomics Databases



Metabolomics Databases

• • Hydrogen-Deuterium Exchange Mass Spectrometry Experiment

  With the development of biopharmaceutical technology, proteins are gradually being applied in various clinical indications as main targets or therapeutic agents in drug research. The structure of proteins is dynamic, and it can change with post-translational modifications, ligand binding, or interactions between proteins. Therefore, studying the conformational changes of proteins in the drug design process is crucial for understanding the mode of action and function of candidate drugs.

• • ECD Circular Dichroism Spectroscopy

  Research on chiral molecules has always held an important position in the fields of physics, chemistry, and biology. Circular dichroism (CD), as a powerful spectroscopic technique, reveals the spatial structure and conformation of molecules by measuring the difference in absorption of chiral molecules to left- and right-circularly polarized light. Circular dichroism can be divided into two types: electronic circular dichroism (ECD) and vibrational circular dichroism (VCD).

• • Detection of Free Thiol Content

  The free thiol, also known as a thiol group or sulfhydryl group, is the -SH group at the end of the cysteine residue side chain. It is highly reactive and can be oxidized by oxidizing agents to form various thiol modifications. The chemical properties of free thiols are very active and can bind to proteins to change their structure and function, or react with oxygen molecules to cause oxidative damage to cells and tissues.

• • How to Detect mRNA Capping Efficiency?

  Messenger RNA (mRNA) is a nucleotide sequence that encodes proteins and consists of five main parts: the 5' cap structure, the 5' untranslated region (UTR) that is associated with translation efficiency, the open reading frame (ORF) region that encodes the protein, the 3' UTR that is associated with translation efficiency, and the poly-A tail composed of multiple adenosine monophosphates, which is related to mRNA stability and translation efficiency.

• • Phosphorylation Modification Proteomics

  Phosphorylation-based modomics is a discipline that focuses on the comprehensive study of phosphorylation modifications in organisms. In the fields of biochemistry and molecular biology, phosphorylation is a critical post-transcription protein modification, playing a core role in regulating protein function, cell signal transmission, metabolic pathways, and various cellular processes.

• • Shotgun Proteomics

  Shotgun proteomics is a technique used for the large-scale identification and quantitation of proteins. This method utilizes mass spectrometry technology and is capable of rapidly and high-throughput analyzing the protein composition within complex biological samples. The process of Shotgun proteomics usually begins with the extraction and digestion of proteins.

• • Proteomic Analysis

  Proteomic analysis is a technique used to study the expression, function, and interactions of all proteins in cells, tissues, or organisms. This analysis is critical for understanding biological processes, disease mechanisms, and the discovery of new drug targets and biomarkers. The main methods and applications of proteomic analysis include: Mass Spectrometry (MS): Most commonly used technique in proteomic research.

• • How to Interpret a Circular Dichroism

  Circular Dichroism (CD) spectroscopy is an analytical technique used to study molecules, particularly large biological molecules such as proteins and nucleic acids. It is based on the difference in absorption of left-handed and right-handed polarized light by molecules. Here is a basic guide to interpreting a circular dichroism spectrum: Understanding the structure of the chart: The horizontal axis (X-axis) typically represents the wavelength (in units of nanometers, nm). The vertical axis (Y-axis)

• • Lactylation Proteomics Analysis

  "Lactylation proteomics analysis" refers to the systematic study of protein lactylation modification. Lactylation refers to the modification of a protein on a specific lysine residue through a reaction with lactate. This is a post-translational modification (PTM). In recent years, with the rapid development of metabolomics and proteomics, it has been discovered that lactate is not only an intermediate product of metabolism but also plays an important role in cell signaling, gene expression regulation

• • Overview of Antibody De Novo Sequencing

  Antibody de novo sequencing is categorized based on the type of antibody samples. We typically describe antibody samples by their "clonality," which refers to the number of different antibody sequences or genetic lineages present in the sample.