Resources

Proteomics Databases



Metabolomics Databases

• • BCA Assay Measures Protein Content

  BCA (Bicinchoninic Acid, Sodium Salt of 2,2'-Biquinoline-4,4'-Dicarboxylic Acid) is a pale yellow powder that is hygroscopic and easily soluble in water or ethanol. BCA forms a stable water-soluble complex that highly specifically binds with Cu+ to produce a purple complex. This complex has a maximum absorbance at 562nm and is directly proportional to the protein concentration. Protein content refers to the total mass or quantity of proteins in a sample, which can be determined using the BCA method.

• • Forward and Reverse Actions in High-Performance Liquid Chromatog

  High Performance Liquid Chromatography (HPLC) can be either Normal Phase HPLC or Reverse Phase HPLC, depending on the nature of the chromatographic column and mobile phase. In Normal Phase HPLC, the stationary phase of the chromatographic column is polar (such as silica gel), while the mobile phase is nonpolar or moderately polar solvent. In this setup, polar molecules have longer retention times in the column because they have stronger interactions with the polar stationary phase.

• • Does Protein Sequencing Require Purification?

  Protein purification is typically necessary before protein sequencing. This is because purified protein samples can reduce potential sequencing errors and improve the accuracy of sequencing results. Therefore, the purification process is an important step in the entire protein sequencing workflow.

• • Proteomics Sequencing Sample Preservation Requirements

  The storage conditions of proteomic sequencing samples have a significant impact on the sequencing results. The main objective of sample preservation is to maintain sample stability and prevent protein degradation and modifications. Here are the basic storage requirements for proteomic sequencing samples.

• • Protein Omics Sequencing Cellular Sample Processing

  Cell sample processing is a crucial step in proteomics research. The main steps involved in processing cell samples are as follows: cell culturing and collection, cell lysis, protein extraction and purification, protein identification and quantification, data analysis.

• • Single Clone Antibody Variable Region Sequencing

  Monoclonal antibodies are highly specific biological molecules that have important applications in various fields such as disease diagnosis, treatment, and biomarker discovery. Monoclonal antibodies can trigger an immune response in the body by recognizing and binding to specific antigens, thus combating pathogens.

• • The Process of Discovering New Antibodies by Using Sequencing

  The identification and study of antibody sequences are important components of modern biomedical research. These antibody sequences can be used to develop vaccines, develop new treatment strategies, and even analyze the workings of the immune system. The basic process of discovering new antibodies through sequencing is as follows.

• • Absolute Quantification of the Proteome

  Proteomic quantitative methods include relative quantification and absolute quantification. Relative quantification, also known as comparative proteomics, refers to comparing and analyzing the relative changes in protein expression levels in different physiological and pathological states of cells, tissues, or body fluids. Absolute quantification of proteomics measures the absolute amount or concentration of each protein in the cell, tissue, or body fluid proteome.

• • Protein Qualitative and Quantitative Analysis

  This passage talks about several commonly used methods for determining the types, structures, and functions of proteins.

• • Isoelectric Focusing Electrophoresis to Determine Protein pI

  Proteins are ampholytes and their charges come from the ionized groups on the side chains of amino acids. These groups can dissociate in solutions at certain pH values, resulting in a net charge on the protein. When a protein solution is at a certain pH, the tendency for the protein to dissociate into positive and negative ions is equal, making it a zwitterion with a net charge of zero. This pH is known as the protein's isoelectric point (pI).