Resources

Proteomics Databases



Metabolomics Databases

• • Considerations for MS Identification of Phosphorylation Sites

  Phosphorylation is a common type of protein post-translational modification (PTM), playing a significant role in biological processes such as cell signal transduction, protein activity and stability regulation. Mass spectrometry is one of the main methods for identifying protein phosphorylation, but the following issues need to be considered in the actual operation:

• • How to Detect the Protein Ubiquitination Level via Western Blot?

  Western Blot Detection of Protein Ubiquitination Level Western blot is a commonly used method for protein detection, which can accurately detect the presence and expression level of specific proteins in samples, as well as post-translational modifications of proteins, such as phosphorylation and ubiquitination. This article will mainly introduce how to use Western blot to detect the ubiquitination level of proteins.

• • Yeast Proteomics Analysis Strategy

  In the field of biotechnology and molecular biology, yeast (especially the brewing yeast Saccharomyces cerevisiae) has become an extremely important model organism due to its ease of cultivation, relatively simple genome, and remarkable similarity to higher organisms. It is not only widely used in industrial production and fermentation processes, but also provides an outstanding model for understanding the biological processes of more complex organisms.

• • AI and Multiplex Cloning Redefine Aantibody Discovery

  Antibodies, as important biopharmaceuticals, play a significant role in the treatment of cancer, autoimmune diseases, and infectious diseases. Antibody discovery, as a crucial process in antibody drug design, has witnessed the application of numerous new technologies. However, antibody research still faces multiple challenges such as affinity, specificity, stability, and immunogenicity.

• • Lipidomics Study of Escherichia Coli Metabolism

  In modern life science research, lipidomics has become an indispensable technique for understanding cell functions, disease mechanisms, and drug action mechanisms. Lipids, as important components in organisms, not only play a key role in the structure and function of cell membranes, but also participate in various biological processes such as energy storage, signal transduction, and cell death.

• • Metabolic Flux Analysis of Blood Cells

  A deep understanding of cellular metabolism is particularly important in modern biomedical research and clinical diagnostics. This is because cellular metabolic processes are directly related to the physiological state of cells, the onset and development of disease, and the response to treatment.

• • CD Spectroscopy of Protein Conformational Changes

  Introduction:In biology, the structure and function of proteins are closely related. Therefore, understanding and observing the conformational changes of proteins are crucial for unraveling their biological functions. This article mainly discusses how to observe and analyze protein conformational changes using circular dichroism spectroscopy.

• • Plant Stem Vein Protein Isoelectric Point Analysis Based on cIEF

  Plant stem vein-based protein isoelectric point analysis based on capillary isoelectric focusing (cIEF) is a refined biotechnology specifically used to investigate the isoelectric points (pI) of proteins within plants. This technique is particularly important in plant science research as it not only reveals the molecular characteristics of proteins but also helps researchers gain a deeper understanding of plant physiological processes.

• • Protein Mass Spectrometry Analysis Experimental Tips for Success

  Mass spectrometry is an important technique in modern proteomics research, with a crucial role in revealing the structure, function, and interaction of proteins. However, the success of mass spectrometry experiments often relies on the skill and experience of the experimenter. This article will share some tips and suggestions for successfully conducting mass spectrometry experiments, helping readers improve experimental efficiency and reduce errors.

• • Protein Purity Analysis in E. coli Using Chromatography Techniqu

  Protein purity analysis of Escherichia coli is a crucial step in biotechnology and molecular biology research, especially in the fields of protein engineering, drug development, and proteomics. This technique primarily utilizes two chromatographic methods, molecular sieving (gel filtration) and reverse-phase chromatography (RP-HPLC), to separate and purify proteins from Escherichia coli, ensuring the obtainment of high-purity protein samples for subsequent structural and functional analysis.