Selected Reaction Monitoring Service

Selected Reaction Monitoring is a highly targeted mass spectrometry (MS) technique designed for the precise quantification of specific peptides and proteins within complex biological mixtures. Unlike traditional MS approaches that analyze broad spectra, Selected Reaction Monitoring focuses on predefined precursor and fragment ion pairs, ensuring exceptional specificity and sensitivity. Selected Reaction Monitoring method is particularly valuable for quantifying low-abundance proteins and validating biomarkers, as it eliminates background noise and enhances signal detection. It is widely regarded as a gold standard in targeted proteomics due to its reproducibility and quantitative accuracy, making it indispensable for advanced protein studies and clinical applications.

 

Selected Reaction Monitoring enables precise quantification of peptides, proteins, and post-translational modifications (PTMs), playing a key role in biomarker discovery, protein interactions, and pharmacoproteomics. Selected Reaction Monitoring also supports therapeutic protein analysis, including stability and glycosylation, and facilitates large-scale proteomic studies, advancing structural biology, disease research, and personalized medicine.

 



 

Picotti, P. et al. Nat Methods. 2012.

 

Figure 1. Examples of Application of Selected Reaction Monitoring in Targeted Proteomics

 

Technical Principles

The technical foundation of Selected Reaction Monitoring (SRM) lies in its ability to target specific ion transitions for each analyte of interest. Using a triple quadrupole mass spectrometer, Selected Reaction Monitoring first isolates the precursor ion of selected specific analyte in the first quadrupole (Q1) and fragments it in the second quadrupole (Q2). Contaminant ions are also isolated and fragmented together with the target ions. In the third quadrupole (Q3), a specific fragment ion from the target analyte (transition) is selected and guided to the detector. The number of target fragment ions is counted over time, resulting in an SRM trace for each transition. Multiple transitions can be monitored in parallel, providing a comprehensive view of the analyte. This process ensures unparalleled specificity by isolating and quantifying only the ions of interest, while minimizing interference from non-target molecules.

 



 

Picotti, P. et al. Nat Methods. 2012.

 

Figure 2. The Principles of Selected Reaction Monitoring Technique

 

Services at MtoZ Biolabs 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. MtoZ Biolabs offers a comprehensive Selected Reaction Monitoring Service, leveraging cutting-edge instrumentation and expertise to deliver precise and reliable results. Our Selected Reaction Monitoring Service includes tailored method development, sample preparation, data acquisition, and detailed data interpretation, specializing in quantifying target peptides, characterizing post-translational modifications, and analyzing protein interactions in their biological context.

 

Service Advantages

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Selected Reaction Monitoring Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. Robust for Large-scale Studies: With its ability to quantify multiple proteins in a single analysis, selected reaction monitoring is well-suited for large-scale studies in structural biology, disease mechanisms, and personalized medicine. 

3. Low Background Noise: By selecting specific precursor and fragment ion pairs, selected reaction monitoring minimizes background noise, ensuring a clear and reliable signal from the analytes of interest. This results in higher signal-to-noise ratios for more accurate results.

4. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

5. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all selected reaction monitoring data, providing clients with a comprehensive data report.

 

Applications

Below are key applications of our Selected Reaction Monitoring Service:

• Quantifying protein abundance in complex biological samples.
• Biomarker discovery and validation for disease research.
• Analyzing protein-protein interactions and dynamics.
• Monitoring drug-target engagement in pharmacoproteomics.
• Assessing post-translational modifications (e.g., phosphorylation, glycosylation).
• Studying therapeutic protein stability and heterogeneity.

 

Case Study

Selected Reaction Monitoring-Based Targeted Assays for the Validation of Protein Biomarkers in Brain Tumors

 



 

Ghantasala, S. et al. Front Oncol. 2021.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files

 

At MtoZ Biolabs, we are committed to providing high-quality Selected Reaction Monitoring Service that accelerate your research progress with reliable and actionable results. Contact us today to learn more!