Sequencing Analysis

• • Unknown Protein Sequence Analysis Service

  Analyzing unknown protein sequences is a critical area in contemporary biological research. Through the examination of amino acid sequences of unknown proteins, scientists can uncover the functions, structures, and interactions of these proteins. Understanding protein sequences is pivotal for disease research, drug discovery, and fundamental biological studies, fostering scientific progress and technological innovation.

• • Glycosylation Protein Analysis Service

  Glycosylation, a critical post-translational modification, plays essential roles in cellular signaling, immune responses, and protein folding and function. At MtoZ Biolabs, we leverage advanced mass spectrometry and bioinformatics to deliver precise analyses of protein glycosylation. Our platform features high-resolution mass spectrometers and sophisticated data analysis software, targeting complex glycan structure identification and quantification.

• • Top-Down Based Glycoprotein Analysis Service

  Glycoproteins are proteins that are covalently bonded with one or more oligosaccharide chains. They are composed of a protein backbone and carbohydrate moieties. The glycan chains are attached via two main types of glycosylation: (1) N-glycosylation: Glycans are attached to the nitrogen atom in the side chain of asparagine residues through N-acetylglucosamine (GlcNAc); (2) O-glycosylation: Glycans are attached to the oxygen atom in the side chain of serine or threonine residues through N-acetylgalactosamine

• • Biopharmaceutical Glycan Analysis Service

  Biopharmaceutical glycans refer to polysaccharide compounds associated with biopharmaceutical products. These compounds are typically attached to proteins or lipids through glycosylation. Glycosylation is a crucial modification in biopharmaceutical products, affecting protein stability, activity, and immunogenicity. The analysis of biopharmaceutical glycans is primarily used to identify and quantify glycosylation modifications in biopharmaceutical products.Analyzing biopharmaceutical glycans ensures the con

• • Antibody N-Terminal Cyclization Analysis Service

  Antibody N-terminal cyclization is a process in which the N-terminal amino acid sequence of an antibody forms a stable cyclic structure through a cyclization reaction. This reaction is typically catalyzed by enzymes such as cyclase, which recognize specific N-terminal sequences. The cyclization alters the antibody’s spatial structure, enhancing stability and function, reducing degradation and inactivation, and improving binding affinity to antigens. Additionally, the unique structure of cyclized antibodies

• • Methylation Protein Analysis Service

  Methylated proteins undergo modifications where methyl groups (-CH3) are added to specific amino acid residues, such as lysine and arginine. This process is catalyzed by methyltransferases, which transfer methyl groups from S-adenosylmethionine (SAM) to the amino acid residues. Methylation can result in mono-methylation (one methyl group), di-methylation (two methyl groups), or tri-methylation (three methyl groups).

• • Glycosphingolipid Analysis Service

  Glycosphingolipids are amphipathic biomolecules with a ceramide skeleton and a polysaccharide head, extensively present in cell membranes. They regulate cell behavior and are pivotal in processes such as cellular communication, disease pathogenesis, and cellular recognition. Alterations in glycosphingolipid levels are associated with neurodegenerative disorders, metabolic imbalances, and certain cancers, highlighting their significance in disease diagnostics and therapeutics. Accurate glycosphingolipid anal

• • Ubiquitinated Protein Analysis Service

  Ubiquitination involves the covalent attachment of ubiquitin molecules to target proteins, following these steps: (1) Activation: Ubiquitin-activating enzyme (E1) activates ubiquitin via ATP hydrolysis. (2) Conjugation: Ubiquitin-conjugating enzyme (E2) transfers the activated ubiquitin to itself. (3) Ligation: Ubiquitin ligase (E3) identifies specific substrate proteins and attaches ubiquitin to the lysine residues of these proteins. This modification tags proteins for degradation, regulates their activity

• • Phosphorylated Protein Analysis Service

  At MtoZ Biolabs, we utilize Thermo Fisher's Orbitrap Fusion Lumos mass spectrometer combined with nano-liquid chromatography to offer integrated phosphorylation protein analysis services.

• • Glycopeptide Analysis Service

  Glycosylation represents one of the most prevalent and functionally significant post-translational modifications in biology. At MtoZ Biolabs, we specialize in innovative glycopeptide analysis services, employing state-of-the-art mass spectrometry to thoroughly investigate glycoproteins' structures and functions. Our offerings extend beyond traditional methods, incorporating the latest scientific discoveries and innovations to elucidate glycosylation's roles in protein expression, disease development