Serum Protein SEC-MALS Molecular Weight Detection

Serum proteins are a category of proteins present in plasma, playing a variety of significant physiological and biochemical functions within the body. These proteins are typically synthesized by the liver and transported to various tissues and organs through the circulatory system. They play crucial roles in maintaining physiological balance, immune responses, carrying biomolecules, drugs, and hormones, etc. The structure and function of serum proteins, as well as their changes during disease processes, are closely related. Therefore, accurately measuring the molecular weight and distribution of serum proteins is of great scientific significance and application value for understanding their biological functions, disease diagnosis, and new drug development. For this purpose, the SEC-MALS (Size-Exclusion Chromatography with Multi-Angle Light Scattering) molecular weight detection technology came into being, becoming an important tool for the study of serum proteins.

 

Serum Proteins

Serum proteins are the total proteins in plasma after removing fibrinogen, including various different proteins like albumin, globulin, coagulation proteins, and others. They play key roles in maintaining plasma osmotic pressure, carrying hormones, vitamins, drugs, and antibodies, as well as in immune response and coagulation mechanisms. Understanding the molecular weight and structure of serum proteins is crucial for disease diagnosis, drug development, and biological research.

 

Introduction to SEC-MALS Technology by MtoZ Biolabs

SEC-MALS is an advanced protein analysis technology, combining Size-Exclusion Chromatography (SEC) and Multi-Angle Light Scattering (MALS) technologies. SEC is used to separate proteins into different size distributions, while MALS measures the scattering intensity of scattered light at different angles, used to measure the absolute molecular weight, size distribution, and shape of macromolecules, for a deeper understanding of the properties and structure of biological macromolecules.

 

1. Size-Exclusion Chromatography (SEC)

SEC is a liquid chromatography technique used to separate macromolecules based on their size distribution. The sample flows through a porous column packing, where larger molecules are excluded as they pass through the column, while smaller molecules stay in the column for a longer time. This results in the molecules distributing in the detector according to their size, achieving molecular separation.

 

2. Multi-Angle Light Scattering (MALS)

MALS is an optical technology used to measure the angle distribution of scattered light from macromolecular particles in solution. By measuring the scattering light intensity at different angles, the molecular weight of macromolecules can be calculated. MALS is often used in conjunction with SEC to achieve high-precision molecular weight determination.

 

Applications

1. Molecular Weight Determination

SEC-MALS technology can accurately measure the molecular weight of serum proteins, helping to understand their conformation and composition.

 

2. Drug Interactions

By measuring the interaction between drugs and serum proteins, scientists can predict the distribution and metabolism of drugs.

 

3. Disease Research

SEC-MALS helps reveal the association between serum proteins and diseases such as cancer, diabetes, and immunological diseases.

 

4. Biomedical Research

Plays a critical role in vaccine development and biomedical research.

 

Serum proteins are one of the important topics in biomedical research, and the SEC-MALS molecular weight detection technology provides us with tools for a deep understanding of these key proteins. MtoZ Biolabs uses Thermo Fisher's Q ExactiveHF mass spectrometry platform, Orbitrap Fusion mass spectrometry platform, Orbitrap Fusion Lumos mass spectrometry platform combined with Nano-LC, to introduce a serum protein analysis technology package, and combined with Size-Exclusion Chromatography (SEC) and Multi-Angle Light Scattering (MALS) technology, it can provide serum protein SEC-MALS molecular weight detection services, for more service details, feel free to inquire!