Single-Molecule Protein Sequencing Service

In the realm of proteomics, understanding protein structure and function at the individual molecule level is key to unlocking the complexities of biology. Traditional protein sequencing methods, such as Edman degradation or mass spectrometry-based shotgun proteomics, often rely on bulk analysis of peptides derived from protein digests. While these methods have been instrumental, they are inherently limited by the averaging effect, where important nuances, such as low-abundance proteins, post-translational modifications (PTMs), and rare protein isoforms, can be overlooked. Single-Molecule Protein Sequencing Service represents a transformative approach, enabling the direct, high-resolution sequencing of intact proteins at the single-molecule level.

 

At MtoZ Biolabs, we provide a state-of-the-art Single-Molecule Protein Sequencing Service based on mass spectrometry (MS), offering an unparalleled level of sensitivity and precision in proteomics analysis. This innovative technique captures data from individual protein molecules, bypassing the need for protein digestion or peptide fragmentation, and allows for the sequencing of intact proteins in their native forms. Furthermore, it offers the ability to sequence proteins that have previously been difficult or impossible to analyze, such as membrane proteins or large, multi-subunit complexes. By focusing on single molecules, Our service provides researchers with the ability to uncover detailed insights into protein sequences, addressing critical challenges that traditional sequencing methods cannot resolve: 

 

1. Sequence Variations

Our service can identify subtle variations between individual protein molecules, which are crucial for understanding disease mechanisms, genetic mutations, or drug interactions that might otherwise be overlooked in bulk analyses.

 

2. Post-Translational Modifications (PTMs)

We offer precise detection of PTMs such as phosphorylation, glycosylation, or acetylation, which can significantly alter protein function, activity, and interactions, providing deeper insights into protein regulation.

 

3. Rare Forms

Our single-molecule approach enables the identification of rare or low-abundance protein forms that are often missed in conventional sequencing due to their infrequent occurrence, ensuring no valuable data is lost.

 

Analysis Workflow

 



 

Service Advantages

1. Advance Analysis Platform

MtoZ Biolabs established an advanced single-molecule protein sequencing service platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

2. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

3. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all single-molecule protein sequencing data providing clients with a comprehensive data report.

 

4. No Amplification Required

Single-molecule protein sequencing analyzes proteins directly, eliminating the need for amplification. This approach minimizes sample preparation steps, reducing the risk of loss or contamination, and ensures that the data reflects the true protein profile without introducing biases from amplification processes.

 

5. No Protein Digestion

Unlike traditional methods that require protein digestion into peptides, single-molecule protein sequencing directly sequences intact proteins. This approach preserves the native structure and sequence, enabling more accurate identification of protein variants. It also provides precise amino acid sequences, even for regions traditionally difficult to analyze, such as disordered domains or transmembrane areas.

 

Applications

1. Disease Mechanism Research

Identifies protein variants and modifications involved in disease pathways, improving understanding of disease mechanisms.

 

2. Biomarker Discovery

Detects novel biomarkers in low-abundance proteins, advancing early disease diagnosis and personalized medicine.

 

3. Drug Development

Unveils protein targets, interactions, and modifications, aiding in the design of more effective therapeutics.

 

Deliverables

1. Experimental procedures

2. Related mass spectrometry parameters

3. Mass spectrometry images

4. Raw data

5. Protein sequence information

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. If you are interested in our Single-Molecule Protein Sequencing Service, please feel free to contact us.