Sucrose Metabolism Analysis

Sucrose, a disaccharide comprising glucose and fructose, has the molecular formula C₁₂H₂₂O₁₁. It is synthesized in numerous plants through enzyme-driven reactions and distributed to tissues like roots, fruits, and nectar. Sucrose undergoes hydrolysis to release energy, supporting plant growth and development.

 

Sucrose biosynthesis involves UDP-glucose and fructose-6-phosphate, catalyzed by sucrose-6-phosphate synthase. Sucrose breakdown primarily involves two enzymes: invertase, which hydrolyzes sucrose into glucose and fructose for use in metabolic processes, and sucrose synthase, which reversibly converts sucrose and UDP to UDP-glucose and fructose.

 

Sucrose serves as a critical node in carbon metabolism across various organisms. The metabolic pathways of sucrose are vital for numerous physiological functions. A thorough understanding of the biochemical pathways and regulatory mechanisms governing sucrose metabolism is crucial for enhancing agricultural practices, advancing biotechnological innovations, and boosting bioenergy production.

 



 

Figure 1. The Structure of Sucrose

 

Services at MtoZ Biolabs

At MtoZ Biolabs, our sucrose metabolism analysis service is based on advanced Mass Spectrometry techniques combined with Liquid Chromatography or Gas Chromatography for the separation of sucrose metabolites. The metabolites are ionized and detected based on their mass-to-charge ratio, allowing us to identify and quantify each metabolite accurately. Furthermore, we employ tandem mass spectrometry (MS/MS) for structural elucidation of the metabolites, providing in-depth insights into the sucrose metabolic pathway. Our team consists of proficient scientists with extensive experience in metabolomics, ensuring the highest quality of data for your research needs.

 

Analysis Workflow

 



 

Applications

Our sucrose metabolism analysis service is applicable in various fields including but not limited to:

• Plant Biology: Understanding sucrose metabolism in plants, which is crucial for their growth and development.
• Medical Research: Studying the role of sucrose metabolism in diseases such as diabetes and obesity.
• Food Science: Analyzing the sucrose content and its metabolism in different food items.

 

Sample Submission Requirements

• Please ensure your submitted sample surpasses 0.2 g or 0.2 mL.
• More than 3 biological replicates should be prepared.
• Samples should be stored in a -80℃ environment and transported on dry ice.
• Please also include a completed sample submission form with your shipment.

 

Let MtoZ Biolabs be your trusted partner in your sucrose metabolism studies. Contact us today to discuss your specific requirements.