SWATH
- High Throughput and Comprehensiveness: Provides extensive quantitative information across the entire proteome.
- Label-Free Quantification: Streamlines experimental procedures and reduces costs.
- High Sensitivity and Reproducibility: Detects low-abundance proteins with high reproducibility.
- Data Reusability: Allows for secondary analysis for various research objectives.
SWATH (Sequential Window Acquisition of All Theoretical Mass Spectra) is a high-throughput, label-free quantitative proteomics technique that combines the benefits of data-dependent acquisition (DDA) and data-independent acquisition (DIA). It allows for comprehensive quantification and identification of proteins in complex biological samples. The essence of SWATH technology lies in DIA, where the mass spectrometer continuously scans all peptide ions within predefined mass windows, rather than targeting specific ions for fragmentation as done in traditional DDA. This makes SWATH particularly suitable for various proteins and peptides, especially those challenging to quantify using conventional methods in complex samples such as plasma, tissues, and cell lysates. This technique is crucial for biomarker discovery, understanding disease mechanisms, validating drug targets, and analyzing biological pathways.
Ludwig, C. et al. Mol Syst Biol. 2018.
Figure 1. The Principle of Sequentially Windowed Data-Independent Acquisition in SWATH-MS
Services at MtoZ Biolabs
At MtoZ Biolabs, we employ SWATH technology for protein quantification. Our service involves configuring the mass spectrometer with multiple consecutive mass windows within a specified mass range, where all ions in each window are fragmented. These ions undergo collision-induced dissociation (CID) to produce fragment ion spectra, which are then matched with a reference library to identify and quantify the peptides and their corresponding proteins.
Analysis Workflow
1. Sample Preparation: Extract and digest proteins to generate peptides.
2. Mass Spectrometry Analysis: Utilize SWATH for data-independent acquisition, producing fragment spectra across multiple mass windows.
3. Data Processing: Use specific software (e.g., OpenSWATH, Skyline) for matching experimental data with a reference library, identifying and quantifying peptides and their corresponding proteins.
4. Result Interpretation: Analyze quantitative results through statistical and bioinformatics methods to elucidate biological significance.
Applications
1. Biomarker Discovery: Critical for identifying diagnostic and prognostic disease markers.
2. Drug Development: Facilitates drug target validation and mechanism studies.
3. Systems Biology: Reveals protein interactions and functional networks in complex biological systems.
4. Clinical Research: Supports personalized medicine and disease studies through high-throughput protein quantification in clinical samples.
Service Advantages
With its high throughput, sensitivity, and reproducibility, SWATH technology has become a vital tool in modern proteomics research. MtoZ Biolabs boasts extensive experience in quantitative proteomics and offers free consultations for more detailed information. Our technical experts are available for complimentary business evaluations.
How to order?
