Tandem Mass Spectrometry Service

Mass spectrometry (MS) accurately measures the mass-to-charge ratios of ionized molecules, enabling their identification, quantification, and characterization. Tandem Mass Spectrometry (MS/MS) represents a sophisticated extension of traditional MS, enhancing its capabilities through a multi-stage analysis process. Tandem Mass Spectrometry involves two or more consecutive mass spectrometry steps, separated by a fragmentation process. In the first stage, precursor ions are selected based on their mass-to-charge ratios. These ions are then fragmented into smaller product ions in the collision cell, and the resulting fragments are analyzed in subsequent MS stages. Tandem Mass Spectrometry approach increases the specificity and sensitivity of analyses, allowing for detailed sequencing, structural elucidation, and identification of proteins and peptides. Tandem Mass Spectrometry Service provides deeper insights and more comprehensive data than single-stage MS alone, making it an invaluable tool for advancing research and applications in proteomics.

 

The fundamental components of an tandem mass spectrometry system include the ionization source, mass analyzers, and fragmentation modules. Commonly used ionization techniques such as Electrospray Ionization (ESI) or Matrix-Assisted Laser Desorption/Ionization (MALDI) generate charged particles from the sample. These ions are then separated and analyzed by mass analyzers like the Orbitrap or Quadrupole-Time of Flight (Q-TOF) instruments. The fragmentation process, achieved through methods such as Collision-Induced Dissociation (CID), Higher-energy Collisional Dissociation (HCD), or Electron Transfer Dissociation (ETD), breaks the precursor ions into smaller fragments that are subsequently detected and analyzed.

 



 

Figure 1. Schematic of Tandem Mass Spectrometry

 

Tandem Mass Spectrometry allows for the analysis of complex proteomes, providing insights into protein expression levels, post-translational modifications (PTMs) such as phosphorylation and glycosylation, and the mapping of protein-protein interactions. It is invaluable for uncovering disease biomarkers, studying cellular signaling pathways, and identifying novel therapeutic targets. Additionally, Tandem Mass Spectrometry aids in the validation of recombinant proteins, the study of protein folding and conformational changes, and the analysis of protein isoforms, making it a versatile tool for advancing both basic and applied proteomics research.

 

Services at MtoZ Biolabs

MtoZ Biolabs specializes in providing cutting-edge Tandem Mass Spectrometry Services, specifically tailored for comprehensive proteomic analysis. Whether you are profiling complex proteomes, identifying novel biomarkers, quantifying protein expression levels, or analyzing post-translational modifications (PTMs), our advanced tandem mass spectrometry technologies deliver precise and reliable results. We offer detailed protein characterization, from peptide sequencing to protein identification and quantification, facilitating insights into protein function, interactions, and dynamics. With a focus on protein science, MtoZ Biolabs empowers researchers to unlock the complexities of biological systems and advance proteomic research to new frontiers.

 



 

Fang, P. et al. Heliyon. 2024.

 

Figure 2. Workflow Diagram for the Detection of Antimicrobial Peptides (AMPs) Through Tandem Mass Spectrometry (MS/MS)

 

Service Advantages

1. Advance Analysis Platform: MtoZ Biolabs established an advanced Tandem Mass Spectrometry Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all tandem mass spectrometry data providing clients with a comprehensive data report.

4. Fast Turnaround Times: Efficient workflows ensuring timely delivery of results.

 

Case Study

Figure 3 illustrates the robust capabilities of tandem mass spectrometry in identifying and quantifying peptides and phosphopeptides. Through MS/MS analysis, the exact location of phosphorylation within a peptide sequence can be pinpointed, even when multiple tyrosine residues are present. Additionally, the peptide TSTIMTDYNPNYC(#)FAGK demonstrates that MS/MS can differentiate between various potential phosphorylation sites within a peptide, confirming that only one amino acid is phosphorylated. Therefore, MS/MS is well-suited for in-depth protein and peptide analysis, providing precise structural information essential for advanced proteomic research.

 



 

Kendrick, N. L. et al. PLoS One. 2020.

 

Figure 3. Identification and Quantification of a Phosphopeptide, and Localization of a Phosphorylation on a Tyrosine Residue within a Peptide

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. If you are interested in our Tandem Mass Spectrometry Service, please feel free to contact us.