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    Targeted Metabolomics Analysis Service

      Targeted metabolomics is a study in which defined metabolites in a sample are identified and quantitatively analyzed. Quantitation and semi-quantitation of defined metabolites are undertaken through the use of internal standard compounds. Targeted metabolomics has an advantage of high specificity and accuracy. Thus, this method has been widely used to analyze and compare multiple targeted metabolites under different physiological states, and is a critical analytical method for discovery of new biomarker in metabolic diseases and study of early diagnosis of diseases.

      MtoZ Biolabs offers targeted metabolomics analysis service using an LC-MS-based multiple reaction monitoring (MRM) and GC-MS-based single ion monitoring (SIM) metabolomics platforms, which have characteristics of high accuracy, specificity, and sensitivity. We guarantee accurate analysis of targeted metabolites, even in low abundance. With our optimized sample preparation methods, interference from high-abundance dominant metabolites can be hugely reduced, thus further increase the detecting sensitivity.

       

      Analysis Workflow

       

      MtoZ Biolabs offers targeted metabolomics analysis service using an LC-MS-based MRM and GC-MS-based SIM technologies, with high accuracy, specificity, and sensitivity.

       

      List of Targeted Metabolites Analysis Services

       

      Targeted Metabolomic Pathways Carbohydrates & Glycolysis Pathways    
      Organic Compounds Acyl CoAs Amino Acid Animal Hormones
      Aldehydes Bioamine Bile Acids
      Carnitines Carotenoids Fatty Acids
      Flavone Flax-Liganans Meat Biomarkers
      Nucleotides Oxysterols Organic Acids
      Plant Hormone Resveratrol Tryptophan Metabolites
      Thiols Vitamins & Coenzymes  
      Inorganic Compounds Metal/Metallomics Polyphenols  

       

      Sample Submission Requirements

      1. Cells and Microbes Samples: 1×10^7cellsor 100 μL/sample. Cellular activities should be terminated instantly, whereas maintaining the cell integrity.

       

      2. Animal Tissues: 100 mg/sample. Collect soft tissues like brain, heart, liver, muscle, and skin. Immediately add preservative reagent and freeze at -80°C.

       

      3. Plant Tissues: 200 mg/sample. Samples should be frozen in liquid nitrogen right after sample collection, and then transferred to -80°C for storage.

       

      4. Serum Samples: Repeated freezing and thawing of sample must be eliminated. Serum samples should be settled down at room temperature for 30 min in the collection tube, and then transferred to centrifuge tube and centrifuged at 8,000 rpm, 5 min. After centrifugation, supernatant is aliquoted to freezing tubes with 200 uL/sample, and stored at -80°C.

       

      5. Urine Samples: 2mL/sample. Urine samples can be aliquoted to centrifuge tubes with 2mL each tube, with addition 1/100 (w/v) sodium azide, and stored at -80°C.

       

      6. Faeces Samples: 200 mg/sample.

       

      Deliverables

      1. Experiment Procedures

      2. Parameters of Liquid Chromatography and Mass Spectrometer

      3. MS Raw Data Files

      4. MS Data Quality Checks

      5. Metabolites Quantification Data

      6. Bioinformatics Analysis (PCA, KEGG, etc.)

       

      Related Services

      Metabolomics

      Targeted Metabolomics

      Untargeted Metabolomics

      Lipidomics

       

      Quantitative Proteomics

      Label-Free

      iTRAQ/TMT

      SILAC/Dimethyl

      SWATH

      MRM

       

      Protein Analysis

      Protein Identification

      Protein Mass Measurement

      PTMs Identification

    • • Carbon Metabolism Analysis Service

      Carbon metabolism analysis in MtoZ Biolabs encompasses both one carbon metabolism and central carbon metabolism. The metabolism of certain amino acids can produce groups containing a carbon, known as one carbon unit (C1 unit). These groups can participate in biosynthesis upon transfer. The metabolic processes involving the production and transfer of C1 unit are referred to as one carbon metabolism. Central carbon metabolism is crucial in the metabolic network

    • • Amino Acids and Their Derivatives Analysis Service

      Amino acids are biologically important organic compounds, composed of amine (-NH2) and carboxylic acid (-COOH) functional groups, along with a side chain attached to each amino acid. Amino acid analysis is a fundamental tool in the analysis of proteins, peptides, and other amino acid-based drugs. Amino acid analysis allows for the quantitative and qualitative analysis of the amino acid composition of samples and enables the determination of atypical amino acids that may be present in proteins and peptides.

    • • Exogenous Metabolite Analysis Service

      Exogenous metabolites refer to external chemical substances, which can be either toxic or harmless. These exogenous metabolites include not only drugs but also dietary supplements, food additives, and environmental pollutants. When exogenous metabolites significantly disrupt the metabolism and transport of endogenous compounds entering the body, they may harm human health.

    • • Unknown Metabolite Identification Analysis Service

      Metabolomics generally divides the credibility of metabolite identification into the following four dimensions: compounds reliably identified (Level 1), compounds obtained through assumed annotations (Level 2), identification of compound categories through assumed annotations (Level 3), and unknown compounds (Level 4).

    • • Ultra-Long Chain Fatty Acid Analysis Service

      Different fatty acids (FAs) vary greatly in the number of double bonds and the length of their carbon (C) chains. Very long-chain fatty acids (VLCFAs) are fatty acids with more than 20 carbon atoms. VLCFAs are precursors of lipid mediators and are components of cellular lipids such as sphingolipids and glycerophospholipids.

    • • Total Trans Fatty Acid Analysis Service

      Fatty acids (FAs) are the main components of lipids, and the physical, chemical, and physiological properties of lipids mainly depend on their fatty acid composition. The composition of fatty acids is determined by gas chromatography of methylated fatty acids. Trans fatty acids are a type of unsaturated fat that occur in small amounts in nature, but have been widely industrially produced from vegetable fats since the 1950s for use in margarine, packaged baked goods, snack foods, and fried fast food.

    • • Bead-Based Multiplex Flow Cytometry Analysis Service

      With the widespread application of flow cytometry (FC) and solid-phase technology, along with the revolutionary advancements in microsphere-based multi-parameter detection technology, FC can qualitatively and quantitatively analyze soluble proteins. Unlike traditional ELISA, which is limited to single-parameter detection, FC multiplex detection can analyze multiple parameters simultaneously from a sample.

    • • Acyl CoAs Analysis Service

      MtoZ Biolabs offers targeted Acyl CoAs analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.

    • • Aldehydes Analysis Service

      MtoZ Biolabs offers targeted aldehydes analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.

    • • Amino Acid Analysis Service

      MtoZ Biolabs offers amino acids analysis service using GC/MS and LC/MS, with high accuracy, specificity, and sensitivity.

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