Targeted Metabolomics Analysis Service
Targeted metabolomics is a study in which defined metabolites in a sample are identified and quantitatively analyzed. Quantitation and semi-quantitation of defined metabolites are undertaken through the use of internal standard compounds. Targeted metabolomics has an advantage of high specificity and accuracy. Thus, this method has been widely used to analyze and compare multiple targeted metabolites under different physiological states, and is a critical analytical method for discovery of new biomarker in metabolic diseases and study of early diagnosis of diseases.
MtoZ Biolabs offers targeted metabolomics analysis service using an LC-MS-based multiple reaction monitoring (MRM) and GC-MS-based single ion monitoring (SIM) metabolomics platforms, which have characteristics of high accuracy, specificity, and sensitivity. We guarantee accurate analysis of targeted metabolites, even in low abundance. With our optimized sample preparation methods, interference from high-abundance dominant metabolites can be hugely reduced, thus further increase the detecting sensitivity.
Analytical Steps of Targeted Metabolomics
1. Data Collection: Targeted Metabolites Standards Are Synthesized and the MRM Transition Values of the Standards Are Measured by Triple Quadrupole MS
2. Quantitative Analysis of Samples Along with the Standards Using Triple Quadrupole MS
3. Statistical Analysis and Bioinformatics Explanation: ANOVA, PCA, KEGG Annotation, etc.
Analysis Workflow
List of Targeted Metabolites Analysis Services
| Targeted Metabolomic Pathways | Carbohydrates & Glycolysis Pathways | ||
| Organic Compounds | Acyl CoAs | Amino Acid | Animal Hormones |
| Aldehydes | Bioamine | Bile Acids | |
| Carnitines | Carotenoids | Fatty Acids | |
| Flavone | Flax-Liganans | Meat Biomarkers | |
| Nucleotides | Oxysterols | Organic Acids | |
| Plant Hormone | Resveratrol | Tryptophan Metabolites | |
| Thiols | Vitamins & Coenzymes | ||
| Inorganic Compounds | Metal/Metallomics | Polyphenols |
Sample Submission Requirements
1. Cells and Microbes Samples: Cellular activities should be terminated instantly, whereas maintaining the cell integrity.
2. Animal Body Fluids Samples: Such as urine, blood, saliva, etc. Samples must be added with anticoagulant and preservative reagents right after sample collection, followed by freezing at -80°C.
3. Plant Tissues: 200 mg/sample. Samples should be frozen in liquid nitrogen right after sample collection, and then transferred to -80°C for storage.
4. Serum Samples: Repeated freezing and thawing of sample must be eliminated. Serum samples should be settled down at room temperature for 30 min in the collection tube, and then transferred to centrifuge tube and centrifuged at 8,000 rpm, 5 min. After centrifugation, supernatant is aliquoted to freezing tubes with 500 uL/sample, and stored at -80°C.
5. Urine Samples: 1 mL/sample. Urine samples can be aliquoted to centrifuge tubes with 1 mL each tube, with addition 1/100 (w/v) sodium azide, and stored at -80°C.
6. Faeces Samples: 10 mg/sample.
Deliverables
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. MS Data Quality Checks
5. Metabolites Quantification Data
6. Bioinformatics Analysis (PCA, KEGG, etc.)
Related Services
Metabolomics
Quantitative Proteomics
Protein Analysis
-
• Carbon Metabolism Analysis Service
Carbon metabolism analysis in MtoZ Biolabs encompasses both one carbon metabolism and central carbon metabolism. The metabolism of certain amino acids can produce groups containing a carbon, known as one carbon unit (C1 unit). These groups can participate in biosynthesis upon transfer. The metabolic processes involving the production and transfer of C1 unit are referred to as one carbon metabolism. Central carbon metabolism is crucial in the metabolic network
-
• Amino Acids and Their Derivatives Analysis Service
Amino acids are biologically important organic compounds, composed of amine (-NH2) and carboxylic acid (-COOH) functional groups, along with a side chain attached to each amino acid. Amino acid analysis is a fundamental tool in the analysis of proteins, peptides, and other amino acid-based drugs. Amino acid analysis allows for the quantitative and qualitative analysis of the amino acid composition of samples and enables the determination of atypical amino acids that may be present in proteins and peptides.
-
• Exogenous Metabolite Analysis Service
Exogenous metabolites refer to external chemical substances, which can be either toxic or harmless. These exogenous metabolites include not only drugs but also dietary supplements, food additives, and environmental pollutants. When exogenous metabolites significantly disrupt the metabolism and transport of endogenous compounds entering the body, they may harm human health.
-
• Unknown Metabolite Identification Analysis Service
Metabolomics generally divides the credibility of metabolite identification into the following four dimensions: compounds reliably identified (Level 1), compounds obtained through assumed annotations (Level 2), identification of compound categories through assumed annotations (Level 3), and unknown compounds (Level 4).
-
• Ultra-Long Chain Fatty Acid Analysis Service
Different fatty acids (FAs) vary greatly in the number of double bonds and the length of their carbon (C) chains. Very long-chain fatty acids (VLCFAs) are fatty acids with more than 20 carbon atoms. VLCFAs are precursors of lipid mediators and are components of cellular lipids such as sphingolipids and glycerophospholipids.
-
• Total Trans Fatty Acid Analysis Service
Fatty acids (FAs) are the main components of lipids, and the physical, chemical, and physiological properties of lipids mainly depend on their fatty acid composition. The composition of fatty acids is determined by gas chromatography of methylated fatty acids. Trans fatty acids are a type of unsaturated fat that occur in small amounts in nature, but have been widely industrially produced from vegetable fats since the 1950s for use in margarine, packaged baked goods, snack foods, and fried fast food.
-
• Bead-Based Multiplex Flow Cytometry Analysis Service
With the widespread application of flow cytometry (FC) and solid-phase technology, along with the revolutionary advancements in microsphere-based multi-parameter detection technology, FC can qualitatively and quantitatively analyze soluble proteins. Unlike traditional ELISA, which is limited to single-parameter detection, FC multiplex detection can analyze multiple parameters simultaneously from a sample.
-
MtoZ Biolabs offers targeted Acyl CoAs analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.
-
MtoZ Biolabs offers targeted aldehydes analysis service using UPLC/TripleQuad5500, with high accuracy, specificity, and sensitivity.
-
MtoZ Biolabs offers amino acids analysis service using GC/MS and LC/MS, with high accuracy, specificity, and sensitivity.
How to order?
