Targeted Quantitative Proteomics SRM
Selected Reaction Monitoring (SRM) is a widely applied technique in quantitative proteomics. Owing to its exceptional sensitivity and quantitative accuracy, SRM is indispensable in various research domains. It is primarily utilized for the quantitative measurement and validation of specific proteins. The operating principle of SRM is based on detecting and quantifying target analytes via predefined target ions.
Operating Principle of SRM
1. Ionization
Initially, the protein sample undergoes ionization, typically facilitated by electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI).
2. Selection
Distinct precursor ions, serving as signature ions of protein molecules, are selected.
3. Fragmentation
The precursor ions undergo collision with gas molecules in the collision cell, generating a series of fragment ions.
4. Detection
Specific fragment ions, referred to as product ions, are subsequently selected for detection.
Applications of SRM
1. Discovery and Validation of Biomarkers
SRM technology enables the rapid and precise validation and quantification of potential biomarkers.
2. Disease Diagnosis and Prognosis
SRM facilitates the detection and quantitative assessment of disease-associated proteins, enabling the evaluation of disease progression and therapeutic efficacy.
3. Identification and Validation of Drug Targets
SRM serves as a powerful tool for identifying and quantifying drug targets, thereby contributing to drug development and optimization.
In summary, targeted quantitative proteomics utilizing SRM is a rigorous and precise technique, exhibiting exceptional sensitivity and accuracy in protein quantification. Nevertheless, certain challenges persist, such as optimizing precursor and fragment ion selection, as well as processing and interpreting quantitative data. Despite these challenges, with continuous technological advancements, SRM is poised to play an increasingly pivotal role in future proteomics research.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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