Terminal Sequencing
Terminal Sequencing is a method used for analyzing the amino acid sequence of protein or peptide molecules, with a particular focus on determining the N-terminal (amino end) and C-terminal (carboxyl end) amino acid sequences of these molecules. The main applications of terminal sequencing technology include protein identification and characterization, protein structure and function research, and biomarker discovery. In drug development, this technology can be used to determine the structure of target proteins, aiding in the design of more effective drug molecules. Terminal sequencing is also used in biotechnology and the food industry for quality control and detection of proteins. For newly discovered proteins, terminal sequencing offers a direct and reliable method to verify their sequence information. By analyzing the N-terminal or C-terminal, researchers can quickly verify the integrity and correctness of protein sequences. In the field of protein engineering, this technology is widely used to confirm the position and type of protein modifications, which is crucial for understanding protein function regulation. Additionally, terminal sequencing is used to study protein degradation pathways, as the identification of terminal sequences of degradation products allows scientists to infer the mechanisms and pathways of protein degradation. In clinical research, terminal sequencing is applied to the study of disease-related proteins, and by analyzing sequence variations of these proteins, scientists can reveal the molecular basis of diseases, providing support for precision medicine.
Analysis Workflow
1. Sample Preparation
The first step is sample preparation. Protein samples need to be purified to ensure the purity and concentration are suitable for subsequent analysis. Typically, samples need to be free of interfering substances, such as salts and other small molecules.
2. Terminal Degradation
After sample preparation is complete, terminal degradation is performed. Common methods include Edman degradation for N-terminal sequencing or Carboxypeptidase digestion for C-terminal sequencing. The degradation process requires strict reaction conditions to ensure sequence accuracy.
3. Data Analysis
Through mass spectrometry or chromatography, the amino acid sequence data after degradation is obtained. Subsequently, bioinformatics tools are used to analyze and match the data, confirming the terminal sequence of the protein.
Experimental Considerations
1. Sample Purity
Ensuring high purity of the sample is key to terminal sequencing. Any impurities may affect the accuracy of degradation and analysis.
2. Reaction Conditions
Strict control of reaction conditions in Edman degradation or Carboxypeptidase digestion, including temperature, pH, and reaction time, is necessary to ensure sequence accuracy.
3. Data Validation
Multiple sequencing runs should be performed to validate the accuracy of data, ensuring the reliability of sequence information obtained.
MtoZ Biolabs is committed to providing customers with high-quality N/C-terminal sequencing analysis services, facilitating breakthroughs in proteomics research. Our offerings are designed to cater to the needs of fundamental research while providing reliable data support for clinical and industrial applications. Partnering with MtoZ Biolabs allows customers to access tailored solutions, guaranteeing the accuracy and dependability of experimental outcomes. We look forward to collaborating with you to advance innovations and developments in proteomics research.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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