Testosterone Analysis Service

Testosterone, primarily secreted by the testes, is the principal androgen and a key steroid hormone in the body. It plays a critical role in the development of male secondary sexual characteristics, maintenance of sexual function, muscle mass formation, bone density preservation, and erythropoiesis. The synthesis of testosterone begins with cholesterol, undergoing a series of enzymatic conversions to pregnenolone and subsequently to testosterone through androstenedione. While the adrenal glands also produce a small amount of testosterone, this is mostly as a precursor for other hormones.

 

In the body, testosterone is predominantly metabolized in the liver, yielding active metabolites such as dihydrotestosterone (DHT) and estradiol. DHT, which exhibits higher biological activity, is primarily involved in the regulation of male characteristics in tissues like the prostate, skin, and hair follicles, whereas estradiol is crucial for maintaining bone health and regulating fat distribution in males. Abnormal fluctuations in testosterone levels are linked to several health conditions, including male infertility, hypogonadism, and certain types of tumors. Furthermore, testosterone remains a significant focus in sports medicine, endocrinology, and anti-aging research. Consequently, precise measurement and analysis of testosterone levels are of paramount importance in the clinical assessment and management of these conditions.

 

Figure 1. Molecular Structure of Testosterone

 

MtoZ Biolabs offers Testosterone Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in testosterone detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity

Detects very low concentrations of testosterone accurately.

 

2. High Specificity

Differentiates testosterone from other compounds using mass spectrometry.

 

3. Rapid Analysis

Efficient sample preparation and fast detection processes for high-throughput analysis.

 

4. High Accuracy

Strict quality control ensures accuracy and reproducibility.

 

5. Versatility

Capable of analyzing testosterone and its related metabolites.

 

Sample Submission Requirements

Make sure the sample volume is greater than 0.2 g or 0.2 mL. For detailed sample submission information, please contact our customer service.

 

Applications

1. For detecting and evaluating conditions related to abnormal testosterone levels, such as male infertility, hypogonadism, and specific tumor types.

 

2. Investigating testosterone's role in metabolism, immune regulation, and other physiological processes.

 

3. Assessing testosterone level changes in aging populations and their implications for age-related health issues.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Testosterone

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into testosterone's extensive physiological roles through top-tier analytical techniques and expert services.