The De Novo Sequencing of Peptides: Unveiling the Overall Picture of Protein Sequence

MtoZ Biolabs is your optimum choice for De Novo Sequencing of Peptides Service, with high-quality and efficient solutions. Peptides are short chain protein molecules formed by the connection of amino acid residues through peptide bonds. They play key functional roles in organisms, including hormones, antibodies, enzymes, and signaling molecules. The specific sequence of peptides determines their structure and function, so accurate sequencing of peptides is very important. This article will introduce the de novo sequencing technology of peptides, reveal the scientific breakthroughs in this field, and its application in the field of biopharmaceuticals.

 

Structure and Significance of Peptides

Peptides are biomolecules formed by the connection of amino acid units through peptide bonds. They are relatively small, flexible, and play important functional roles in organisms. The sequence of peptides determines their three-dimensional structure and interaction methods, which in turn affect their functional performance.

 

Principles and Methods of De Novo Sequencing

De novo sequencing is a technique to determine the sequence of peptides. In the past, this task was very challenging, but with the development of modern science and technology, researchers have made important breakthroughs. The principle of de novo sequencing is based on inferring the sequence of the entire peptide by analyzing the amino acid composition and order of peptide fragments.

 

1. Trial and Error Method

The trial and error method is one of the earliest methods used for de novo sequencing. By repeatedly shortening the peptide step by step at the known sequence end and then determining the amino acid at each truncation point, the complete sequence of the peptide can be inferred. However, this method is inefficient and time-consuming, and its application range is limited.

 

2. Mass Spectrometry Method

Mass spectrometry is one of the commonly used methods for de novo sequencing of modern peptides. By measuring the mass-to-charge ratio of peptide fragments with a mass spectrometer, and then inferring the complete sequence of the peptide by calculation and comparison with known amino acid sequences in the database. The mass spectrometry method is fast and efficient, and can handle larger peptide molecules.

 

3. Gene Sequencing Method

Gene sequencing is another method used for de novo sequencing of peptides. This method determines the sequence of peptides by cloning and sequencing the corresponding genes. Gene sequencing is usually applied when the gene coding region of a known peptide has not yet been resolved.

 

Importance of De Novo Sequencing of Peptides in Drug Development

As an important part of biopharmaceuticals, de novo sequencing has important application value in drug development.

 

1. New Drug Discovery

De novo sequencing technology of peptides can help researchers discover new peptide drugs. By sequencing and analyzing unknown peptides produced in organisms, researchers can find new drug candidates with potential therapeutic effects.

 

2. Drug Design and Optimization

De novo sequencing of peptides can reveal the relationship between their structure and function, which is conducive to drug design and optimization. By understanding the accurate sequence of peptides, researchers can change their structure, improve their drug properties, and enhance their efficacy and stability.

 

3. Drug Safety Assessment

De novo sequencing technology of peptides can help assess the safety of drugs. By determining the variation or mutation in the peptide sequence, researchers can identify potential toxic side effects and drug interactions, and conduct corresponding safety assessments.

 

De novo sequencingof peptides is an important technology that can help researchers restore the full picture of protein sequences. Through de novo sequencing, we can gain a deeper understanding of the structure, function, and interactions of peptides, and promote the progress of biopharmaceutical research and development.