Thermal Stability Analysis of Recombinant Protein Vaccines

Recombinant protein vaccines are a type of vaccine that do not contain complete pathogens and are prepared by specific protein antigens produced by heterologous expression systems. The common heterologous expression systems include bacteria, mammalian cells, plant cells, and insect cells. The appropriate heterologous expression system needs to be selected according to the type of antigen produced. Due to its advantages of good safety, strong stability, and low cost, recombinant protein vaccines have been widely favored by researchers in recent years. The thermal stability of protein drugs refers to the stability of its structure and function under high temperature conditions. Thermo-stability studies of protein drugs can help us understand the structure and function of protein drugs. Moreover, studying the thermal stability of protein drugs plays a crucial role in ensuring its stability during preparation, storage, and transportation.

 

Currently, the common methods for analyzing the thermal stability of recombinant protein vaccines include Differential Scanning Calorimetry (DSC), Circular Dichroism (CD), fluorescence spectroscopy, and Isothermal Titration Calorimetry (ITC). Among them, DSC is the gold standard technology for characterizing protein thermal stability, used for measuring the thermodynamic property changes of proteins during the heating process. DSC experiments can provide information such as the melting point (Tm) and heat capacity (Cp) of proteins to assess their thermal stability.

 

MtoZ Biolabs provides drug quality research services that comply with global pharmaceutical regulations. We offer a one-stop service for recombinant protein vaccine thermal stability analysis. Just tell us your experimental purpose and send us the sample, MtoZ Biolabs is responsible for all subsequent projects, including sample processing, machine analysis, data analysis, and project reports.