Thiols
- Expertise in Thiol Metabolomics: Our team has extensive experience in metabolomics and is skilled at handling complex matrices.
- Advanced Analytical Techniques: We employ state-of-the-art LC-MS/MS for high sensitivity and specificity.
- Customizable Service: We customize our services according to the specific needs of your project.
- High-quality Results: We strive for accuracy and precision in our measurements.
- Metabolic Pathway Studies
- Biomarker Discovery
- Drug Metabolism Studies
- Nutritional and Environmental Studies
Thiols, also known as mercaptans, are organic compounds that contain a sulfhydryl (-SH) group. These compounds play vital roles in biological processes, including enzymatic reactions, protein structure stabilization, and cellular signaling. However, the aberrant metabolism of thiols can lead to various pathological conditions such as oxidative stress, neurodegeneration, and cancer.
Analysis Workflow
Sample Preparation: Thiols are extracted from the sample using appropriate solvents and precipitating agents. The extract is then purified to remove interfering substances.
Derivatization: To improve detection, thiols are derivatized to form more stable and volatile derivatives.
LC-MS/MS analysis: Derivatized thiols are separated using liquid chromatography (LC) and quantified using tandem mass spectrometry (MS/MS).
Data Analysis: The raw data obtained from LC-MS/MS analysis is processed and analyzed to identify and quantify thiols.
Figure 1. Workflow of Thiols Analsis by HPLC-MS/MS
Service Advantages
Applications
Sample Submission Requirements
Blood Samples, Bile: 10 mL
Various Tissues: 10 mg
Faeces: 10 mg
Please transport samples on Dry ice.
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Thiols
4. Mass Spectrometry Images
5. Raw Data
Contact us today to learn more about our Thiol Targeted Metabolomics services and how they can benefit your research.
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