TMT Technology in Mass Spectrometry

TMT (Tandem Mass Tag) technology is a major method for studying protein quantification. This technology realizes quantification by labeling isotopes at the protein or peptide level and using mass spectrometry detection, which enables comprehensive and accurate detection of the expression levels of proteomes in biological samples.

 

How to Analyze Proteomes with Mass Spectrometry TMT Technology

In the experimental process, we first need to extract the proteins in the sample and break them down into peptides. Then, these protein peptides are labeled, which is the core of TMT technology. Subsequently, the labeled samples are analyzed through liquid chromatography and tandem mass spectrometry technology, ultimately obtaining the abundance information of proteins.

 

Features and Applications of Mass Spectrometry TMT Technology

Mass spectrometry TMT technology, with its high throughput, high sensitivity, and high repeatability, holds broad application prospects in proteomics research, drug discovery, biomarker research, and other fields.

 

Moreover, mass spectrometry TMT technology is widely used in various fields such as disease mechanism research and biological evolution research, due to its ability to compare protein expression differences among multiple samples at the same time.In essence, the emergence of mass spectrometry TMT technology allows us to understand the biochemical state of organisms under specific conditions in a more comprehensive and precise manner.