Trimethylamine N-Oxide Analysis Service

Trimethylamine N-oxide (TMAO) is a significant metabolite produced from dietary choline and carnitine by gut microbiota. This compound is present not only in humans but also in marine organisms such as fish and shellfish, where it helps to regulate osmotic pressure in high-pressure deep-sea environments. Recent research has highlighted TMAO's involvement in cardiovascular diseases, renal dysfunction, and other metabolic disorders, making it a key focus of scientific inquiry.

The metabolism of TMAO involves its conversion from trimethylamine (TMA) by the liver, following its production by gut microbiota. Changes in TMAO levels can provide insights into gut microbiota and liver function, serving as a crucial biomarker for both research and clinical diagnostics. Accurate analysis of TMAO is essential for understanding its role in disease mechanisms and for developing targeted treatment strategies.

Figure 1. Molecular Structure of Trimethylamine N-Oxide

MtoZ Biolabs offers Trimethylamine N-Oxide Analysis Service using liquid chromatography-tandem mass spectrometry (LC-MS/MS). This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of TMAO in complex biological matrices.

Analysis Workflow

Service Advantages

Sample Submission Requirements

Sample Types

Serum, plasma, urine, tissue homogenates, and other biological samples.

Sample Volume

At least 200 μL of liquid sample or 200 mg of tissue sample.

Sample Preservation

Store and transport samples at -80 °C.

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on TMAO

4. Raw Data

5. Custom Analysis Report

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into TMAO's extensive physiological roles through top-tier analytical techniques and expert services.