Tryptophan Analysis Service
- Liquid Chromatography-Mass Spectrometry (LC-MS)
- Gas Chromatography-Mass Spectrometry (GC-MS)
- Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
- Rapid Analysis Cycle and Cost-Effectiveness
- Stable, Reliable, and Highly Sensitive Results
- Comprehensive One-Stop Service for Extensive Testing Needs
- User-Friendly Operation with Wide Application Scope
- Supported by Extensive Bioanalytical Resources and Advanced Platforms
Tryptophan (Trp) is a unique and essential amino acid containing an indole structure, primarily existing in the L-form in humans and animals. Since it cannot be synthesized by the body, it must be obtained exclusively through dietary intake. Tryptophan plays a crucial role in protein biosynthesis and serves as a precursor for several important bioactive compounds. The levels of tryptophan in the body depend on dietary intake and the activity of various metabolic pathways.
Tryptophan metabolism in humans is primarily governed by three key pathways: the kynurenine (Kyn) pathway, the 5-hydroxytryptamine (5-HT) pathway (also known as the serotonin pathway), and the indole pathway. These pathways are intricately linked to various diseases and have significant implications for regulating multiple physiological functions, including inflammation, immune response, and neural activity. Understanding these pathways and their related metabolites is essential for research in fields such as neurobiology, immunology, and metabolic disorders.
Xue C.et al.Cell Metab. 2023.
Figure 1. Overview of Tryptophan Metabolism Via the Kyn, 5-HT, and Indole Pathways
MtoZ Biolabs utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to conduct detailed quantitative and qualitative analyses of tryptophan and its metabolites. This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of tryptophan in complex biological matrices.
Analysis Workflow
Experimental Instruments
Service Advantages
Sample Submission Requirements
For detailed sample submission instructions, please consult our technical team.
Applications
1. Clinical Research: Monitor tryptophan levels to study its role in depression, anxiety, and other mental health conditions, aiding diagnosis and treatment.
2. Nutritional Assessment: Analyze tryptophan content in foods and supplements to evaluate nutritional value and guide dietary intake and supplementation strategies.
3. Sleep Research: Assess the role of tryptophan in melatonin synthesis and its potential applications in regulating the sleep-wake cycle.
4. Drug Development: Explore the potential applications of tryptophan and its metabolites in new drug development, particularly for treating mental health and sleep disorders.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Metabolites
4. Raw Data
5. Custom Analysis Report
MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into tryptophan's extensive physiological roles through top-tier analytical techniques and expert services.
How to order?
