Tryptophan Metabolites
- Biomarker Discovery for Disease Diagnosis or Prognosis
- Drug Metabolism and Pharmacokinetics Studies
- Nutritional and Dietary Studies
Tryptophan (Trp) is an essential aromatic amino acid that plays a key role in synthesizing proteins and bioactive molecules, such as serotonin, melatonin, and niacin. It is vital for various physiological processes, including mood regulation, immune response, and sleep. The gut microbiome significantly influences human health, particularly through its role in modulating tryptophan metabolism. It regulates three primary metabolic pathways of tryptophan: the serotonin pathway, the kynurenine pathway, and the indole derivatives pathway. Disruption in these pathways is closely associated with several health conditions, including inflammatory bowel disease, irritable bowel syndrome, metabolic syndrome (such as diabetes, obesity, NAFLD, and insulin resistance), cardiovascular diseases, and neuropsychiatric disorders like anxiety, depression, and autism.
Hsu, C, N. et al. Int. J. Mol. Sci. 2020.
Figure 1. Overview of Tryptophan Metabolism Through the Kynurenine, Serotonin, and Indole Metabolic Pathways
MtoZ Biolabs offers advanced Tryptophan Metabolites Analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS). This high-precision technology ensures accurate quantification of tryptophan and its metabolites in complex biological samples, providing critical insights into metabolic processes and disease mechanisms.
Services at MtoZ Biolabs
At MtoZ Biolabs, we utilize advanced mass spectrometry techniques to provide a comprehensive tryptophan metabolites profiling service.
Analysis Workflow
We use an optimized sample preparation protocol, coupled with LC-MS/MS analysis, to accurately profile and quantify tryptophan metabolites in various biological samples. The workflow includes sample collection, extraction, LC-MS/MS analysis, data processing and statistical analysis.
Figure 2. Workflow of Tryptophan Metabolites Profiling by LC-MS/MS
Service Advantages
1. Advanced Techniques: We use state-of-the-art LC-MS/MS for highly sensitive and reliable detection of tryptophan metabolites.
2. Comprehensive Analysis: Not only tryptophan, but also its downstream metabolites, including serotonin, melatonin, kynurenine, and others, can be simultaneously analyzed.
3. Qualified Team: Our team of experts ensures high-quality data and accurate interpretation.
4. Customizable Service: Our service can be tailored to meet your specific research needs.
Applications
Sample Submission Requirements
For other sample types and volume, please contact us.
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Tryptophan Metabolites
4. Mass Spectrometry Images
5. Raw Data
At MtoZ Biolabs, we are dedicated to providing top-quality metabolomics services to facilitate your research. Our experienced team can guide you throughout the process from experiment design to data analysis, ensuring your project's success.
How to order?
