Ubiquitin-proteomics

Ubiquitination is a crucial protein modification process within cells that involves covalently attaching ubiquitin molecules to target proteins through a series of enzymes (E1, E2, E3). This process regulates protein degradation, signal transduction, protein-protein interactions, and cellular localization. Different types of ubiquitin chains, including K48 and K63 chains, play various roles in cellular functions, involving key biological processes such as protein degradation and signal transduction.

 



Woo, S. M. et al. BMB Rep. 2019.

Figure 1. The Process of the Ubiquitin Proteasome System (UPS)

 

Ubiquitin-proteomics utilizes high-resolution mass spectrometry to systematically analyze ubiquitination sites within the proteome and their dynamic changes. MtoZ Biolabs offers professional ubiquitin-proteomics service to help scientists fully understand the biological significance of ubiquitination and its role in diseases.

 

Analysis Workflow

 



 

Service Advantages

 



 

Sample Submission Requirements

 



For detailed sample submission instructions, please consult our technical team.

 

Applications

1. Cell Cycle Research: Reveal the regulatory role of ubiquitination in cell division and proliferation.

 

2. Signal Transduction: Analyze ubiquitination of key proteins in signaling pathways to understand their functions.

 

3. Disease Mechanism Research: Identify disease-related ubiquitination sites and proteins through ubiquitin-proteomics, aiding in disease mechanism studies and new drug development.

 

4. Drug Screening: Evaluate the impact of drugs on ubiquitination, supporting drug screening and target validation.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Ubiquitinated Proteins

4. Mass Spectrometry Images

5. Raw Data