Urea Cycle Metabolism Analysis
The urea cycle, also known as the ornithine cycle, is a crucial metabolic pathway in the liver that converts ammonia into urea, which can then be excreted from the body. This cycle is vital for the detoxification of ammonia, a byproduct of protein metabolism, preventing its accumulation to toxic levels. Understanding the urea cycle's intricacies is essential for both human health and plant physiology, given its significant role in nitrogen metabolism.
The urea cycle involves a series of biochemical reactions that occur primarily in the liver. The cycle begins with the conversion of ammonia and bicarbonate into carbamoyl phosphate, catalyzed by the enzyme carbamoyl phosphate synthetase I (CPSI). Carbamoyl phosphate then combines with ornithine to form citrulline through the action of ornithine transcarbamylase (OTC). Citrulline is transported to the cytosol, where it reacts with aspartate to form argininosuccinate, catalyzed by argininosuccinate synthetase (ASS). Argininosuccinate is subsequently cleaved by argininosuccinate lyase (ASL) to produce arginine and fumarate. Finally, arginase (ARG) hydrolyzes arginine to produce urea and regenerate ornithine, completing the cycle.
Figure 1. Schematics of Canonical Urea Pathway
At MtoZ Biolabs, we offer comprehensive Urea Cycle Metabolism Analysis services utilizing advanced mass spectrometry and cutting-edge methodologies.
Technical Principles
Urea cycle metabolism analysis at MtoZ Biolabs is mainly conducted by liquid chromatography–mass spectrometry (LC-MS). The advanced mass spectrometer facilitates incredibly high-resolution analysis, providing comprehensive and accurate profiling of urea cycle metabolites. The technical principles behind LC-MS allow for the separation and identification of these metabolites based on their specific mass-to-charge ratio.
Analysis Workflow
Service Advantages
1. Advanced Technology
State-of-art equipment and software provide high-quality urea cycle metabolism analysis.
2. Experienced Team
A team of experts with rich experience in metabolomics ensures reliable and accurate data.
3. Customizable Service
Customizable service to meet your unique research needs.
4. Comprehensive Report
Reports include not only raw data but also detailed analysis and professional interpretation.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Urea Cycle Metabolism Analysis
4. Raw Data
5. Custom Analysis Report
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