What Are the Main Functions of Olink Proteomics?
Olink proteomics is an advanced biotechnology that enables high-throughput, high-sensitivity, and high-specificity quantitative analysis of specific proteins in biological samples. This is achieved using the innovative Proximity Extension Assay (PEA) technology.
Core Technology and Advantages
The central technology behind Olink proteomics is the PEA technique, which involves the specific recognition and binding of antibodies to target proteins, followed by the amplification of oligonucleotide sequences located at the ends of these antibodies. For each target protein, Olink designs a pair of antibodies that are conjugated with unique single-stranded DNA sequences, each carrying a specific nucleotide probe. Upon binding to the target protein, the complementary DNA probes on the antibodies pair together at their 5’ ends, forming a double-stranded DNA template in the presence of a DNA polymerase enzyme. The resulting DNA signal is then quantified using qPCR or high-throughput sequencing. The intensity of the DNA signal is directly proportional to the concentration of the target protein in the sample, allowing for precise quantification.
The Advantages of Olink Proteomics
1. High Sensitivity
The technology can detect proteins at extremely low concentrations, making it suitable for the analysis of trace amounts in clinical samples. For example, it can detect Interleukin-8 (IL-8) at concentrations as low as 30 fg/ml.
2. High Throughput
Multiple proteins (up to thousands) can be analyzed in parallel in a single experiment, with a minimal sample volume requirement (only a few microliters).
3. High Specificity
The dual-antibody binding and DNA-based detection significantly reduce cross-reactivity, ensuring high data accuracy.
The Applications of Olink Proteomics
1. Early Disease Diagnosis
Olink proteomics can detect disease biomarkers in blood, enabling early diagnosis. For example, in bladder cancer research, proteins such as AREG, ESM-1, and FGFBP1 have been identified as potential biomarkers, providing valuable insights for early detection.
2. Biomarker Discovery
The technology accelerates the discovery of new biomarkers and contributes to the development of personalized medical treatments. Researchers can use Olink proteomics for large-scale biomarker screenings, identifying proteins linked to specific diseases or biological processes.
3. Drug Development
Olink proteomics aids in the rapid screening and validation of drug targets, supporting drug development efforts. Researchers can assess how potential drugs affect target proteins, optimizing drug design.
4. Disease Mechanism Research
The technology can be used to study the molecular mechanisms underlying disease progression. By tracking changes in protein levels during disease development, researchers can gain insights into disease pathogenesis.
5. Market Position and Future Prospects
Olink proteomics technology holds a significant position in the biotechnology sector due to its unique advantages and broad applications. It is widely used in basic research, pharmaceutical development, and clinical trials, spanning various life sciences and disease areas. With continued advancements in the technology and expanding applications, Olink proteomics is poised to demonstrate its immense potential and value across diverse fields.
Olink proteomics represents a biotechnology with vast potential for improving life science research and advancing human health, thanks to its unique technical advantages and wide-ranging applications.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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