What Information About Peptides Can Be Directly Obtained From Mass Spectrometry Detection

Mass spectrometry is a crucial analytical technique used to determine the mass and structural composition of chemical substances. In biochemistry, it is particularly useful for peptide analysis, providing insights such as:

 

1. Peptide Mass

By measuring their mass-to-charge ratios, mass spectrometry can determine peptide masses and deduce their amino acid sequences.

 

2. Peptide Fragment Ions

Secondary mass spectrometry allows for the analysis of peptide fragments, facilitating the deduction of peptide sequences.

 

3. Chemical Modifications in Peptides

Assessing the mass-to-charge ratios of modified peptides reveals details on chemical modifications like phosphorylation and acetylation.

 

4. Peptide Charge State

The technique also provides data on the charge states of peptides, aiding in structural inference.

 

5. Structural Information of Peptides

Tertiary mass spectrometry offers partial structural insights into peptides.

 

MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.

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