X-Ray Powder Diffraction Analysis of Peptide Drug

Peptide drugs are bioactive molecules formed by the connection of multiple amino acids through peptide bonds. Typically, they are composed of 10~100 amino acids, with a relative molecular mass below 10,000. Most peptide drugs originate from endogenous peptides or natural peptides, therefore they have no side effects or minimal side effects on the human body. Compared to protein drugs, peptide drugs also have advantages such as good stability, high purity, low production cost, and low immunogenicity. With the rapid development and maturity of peptide synthesis technology, peptide drugs have become the focus of biopharmaceutical research in recent years. At present, peptide drugs have been widely used in the diagnosis and treatment of tumors, autoimmune diseases, and certain cardiovascular diseases and diabetes, and have broad application prospects.

 

Up to now, the main methods for determining the spatial structure of peptide drugs are: X-ray diffraction (XRD), nuclear magnetic resonance technology (NMR), and electron microscopy technology. Among them, X-ray diffraction is one of the most accurate methods for determining protein structure, and more than 80% of the known protein structures are obtained by this method. X-ray diffraction includes X-ray single crystal diffraction and X-ray powder diffraction, the latter of which has a wider range of detection objects and can be crystalline or non-crystalline solid samples.

 

Based on our existing technology platform, MtoZ Biolabs provides you with one-stop peptide drug X-ray diffraction measurement services. By obtaining cell parameters, such as interatomic distances, ring plane distances, and dihedral angles, we can determine the crystal form and structure of peptide drugs.