Acylation Quantitative Proteomics Service

Acylation is a pivotal post-translational modification influencing how proteins interact, signal, and function within cells. This process, extending beyond simple acylation to include complex forms like propionylation and succinylation, is crucial for understanding how proteins regulate cellular and metabolic pathways. At MtoZ Biolabs, our acylation quantitative analysis service dives deep into these modifications to uncover their roles in health and disease, providing essential insights for identifying disease markers and developing novel therapeutics.

 

Services at MtoZ Biolabs

Utilizing cutting-edge nanoLC-MS/MS technology, MtoZ Biolabs offers a full suite of acylation analysis services, from protein sample preparation to sophisticated data analysis:

1. Quantitative analysis of lysine, serine, and threonine acylation.

2. Detailed assessment of various acylation types, enhancing the understanding of their specific functions and implications in diseases.

3. Identification of modification sites and analysis of protein interactions influenced by acylation.

 



Figure 1. Acylation Quantitative Proteomics Analysis

 

Analysis Workflow

1. Protein Extraction

We extract proteins from a wide range of biological samples, maintaining their integrity and biological activity.

2. Protein Digestion

Proteins are meticulously digested into peptides for detailed analysis.

3. Peptide Enrichment and Separation

We employ targeted enrichment to isolate acylated peptides, simplifying complex samples for precise analysis.

4. Mass Spectrometric Analysis

Using state-of-the-art LC-MS/MS, we quantitatively analyze peptides and accurately identify acetylation sites.

5. Bioinformatics Insights

Our advanced software tools deliver a thorough interpretation of the data, highlighting the significance of each acylation modification.

 

Service Advantages

1. Expertise and Precision: Our specialized enrichment techniques ensure high coverage and detailed identification of acylation sites.

2. Comprehensive Capabilities: We handle a wide range of acylation modifications, providing insights into both common and rare modifications.

3. Efficient and Reliable Results: Our services are designed for speed and accuracy, delivering results within a week.

4. Tailored Solutions: We customize our services to meet the specific needs of each research project, ensuring that every client's scientific questions are thoroughly addressed.

 

Sample Submission Requirements

1. Solid Samples: Minimum of 5 grams for plant tissue, 1 gram for animal tissue.

2. Liquid Samples: At least 1 milliliter of blood, 0.5 milliliters of serum, 10 milliliters of urine.

3. Cell and Microbial Samples: A minimum of 1×10^8 cells, or 400 milligrams dry weight for microbes.

4. Protein Samples: At least 1 milligram required.