Antibody Light and Heavy Chain Variable Region Sequencing Service

Antibodies are Y-shaped molecules composed of two heavy chains and two light chains , each containing constant regions (CR) and variable regions (VR). The VRs are crucial for determining the specificity and affinity of antibodies, mainly consisting of three complementarity-determining regions (CDRs) and four framework regions (FRs). The diversity and specificity of antibodies are primarily determined by the amino acid sequences of the CDRs, which play an essential role in antigen recognition and binding. MtoZ Biolabs specializes in providing high-precision sequencing services for the VRs of antibody light chains and heavy chains, helping researchers and clinicians gain deep insights into antibody structure and function.

 

MtoZ Biolabs uses Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with nano-liquid chromatography to deliver precise sequencing services for the VRs of antibody light chains and heavy chains. Our platform operates independently of pre-existing databases, capable of identifying and sequencing antibodies from various species, ensuring high-precision sequencing even for samples without genomic data.

 

Service Advantages

High Efficiency: Utilizing LC-MS/MS technology, each site is covered at least four times to ensure complete sequence coverage.

 

Low Time Cost: The efficient sequencing process significantly reduces research and development time.

 

Low Sample Requirement: Only 100 μg of antibody per sample are needed, suitable for projects with limited sample availability.

 

High Accuracy: Light chain sequencing error range is +/- 1.2 Da, and heavy chain sequencing error range is +/- 1.8 Da, ensuring high accuracy.

 

Customized Service: Based on customer needs, we can sequence only the light chain, heavy chain, or variable region, or perform full-length sequencing.

 

Applications

1. New Drug Development

By analyzing the VRs of antibody light chains and heavy chains, new anti-cancer targets can be identified, optimizing the design of immunotherapy drugs such as anti-PD-1 and anti-CTLA-4 antibodies.

 

2. Disease Diagnosis

Sequencing specific antibody markers in diseases like systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) helps develop early diagnostic tools, aiding in the early identification and monitoring of disease progression.

 

3. Biotechnology Research

In studying infectious diseases such as COVID-19, sequencing pathogen-specific antibodies reveals immune response mechanisms across species, supporting the development of vaccines and treatment plans.

 

4. Antibody Engineering

Providing VR sequence information supports antibody modification and humanization, enhancing their affinity and stability, and reducing patient immune rejection, as in humanized mouse monoclonal antibodies.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Antibody Light Chain and Heavy Chain Variable Region Sequences

4. Mass Spectrometry Images

5. Raw Data