Biopharmaceutical N-Terminal Sequencing Service

N-terminal sequencing identifies the amino acid sequence initiating from the N-terminal of proteins, crucial for confirming protein identity, understanding functional mechanisms, and evaluating therapeutic and safety impacts of post-translational modifications. The N-terminal sequence can influence protein stability, activity, and biomolecular interactions, essential for consistent therapeutic protein production and compliance with strict regulatory standards.

 



Nakajima, C. et al. Analyst. 2011.

Figure 1. The Workflow of N-terminal De Novo Sequencing of Nα-blocked Proteins by Mass Spectrometry

 

Services at MtoZ Biolabs

In biopharmaceuticals, N-terminal sequencing is pivotal. At MtoZ Biolabs, we employ the Edman degradation method, known for its precision in sequentially identifying amino acids by removing one residue at a time from the N-terminal. We also enhance our sequencing with advanced mass spectrometry, especially valuable for proteins with complex structures or high molecular weights.

 

Analysis Workflow

1. Sample Preparation: Protein purification and preparation under stringent conditions to maintain integrity and prevent degradation.

2. Edman Degradation Sequencing: Cyclic chemical reactions on the protein's N-terminal to cleave and identify each amino acid.

3. Mass Spectrometry Analysis: Secondary sequence confirmation when needed.

4. Data Analysis and Reporting: Expert interpretation of data with comprehensive reports on sequence accuracy, modifications, and anomalies.

 

Applications

1. Quality control in protein pharmaceutical production

2. Protein identification in complex mixtures

3. Characterization of protein modifications and impurities

 

Service Advantages

Expertise: Our experts have deep knowledge in proteomics and biopharmaceutical analytics.

Precision: We utilize of cutting-edge technologies for unmatched accuracy and reliability.

Customization: Our tailored services to meet specific client demands and regulatory requirements.

Comprehensive Support: We offer experimental design to detailed reporting and consultancy.

 

Through MtoZ Biolabs' N-terminal sequencing service, we aim to bolster your biopharmaceutical development by merging precision with innovation, ensuring accurate evaluation and effectiveness of biopharmaceuticals.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Biopharmaceutical N-Terminal Sequencing

4. Mass Spectrometry Images

5. Raw Data