Does a Fold Change Threshold of 1.5 Enhance Acceptance in Proteomics Differential Protein Screening?

In proteomics, selecting a fold change (FC) threshold of 1.5 is commonly used to identify differentially expressed proteins. However, the optimal FC threshold can vary depending on the specific study design, sample size, and research objectives. For instance, studies with smaller sample sizes may require more stringent thresholds to ensure reliability, while larger studies might adopt more lenient criteria to detect a broader range of differential proteins.

 

Additionally, combining FC thresholds with appropriate statistical methods, such as p-value adjustments, is crucial to minimize false positives and ensure the biological significance of the findings.

 

MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.

Related Services

Differential Protein Analysis Service