Edman Based Protein Sequencing Service

The Edman degradation method, proposed by Pehr Edman in 1950, is a protein sequencing technique that determines the amino acid sequence of a protein by sequentially removing and identifying the N-terminal amino acids of a polypeptide chain. The Edman degradation method is significant in protein research. It provides precise determination of the N-terminal amino acid sequence of proteins, particularly suitable for short peptides and purified proteins. It helps verify the translation start sites of proteins, ensuring accurate sequence information. By understanding the N-terminal sequence of proteins, it is possible to infer functional domains and active sites.

 



Shi, Q. et al. Angew Chem Int Ed Engl. 2024.

Figure 1. The Workflow of Edman Based Protein Sequencing

 

MtoZ Biolabs establishes Edman-based protein sequencing platform offering unmatched advantages in N-terminal sequence analysis. The platform is suitable for protein sequence research and proteomics research. We also provide mass spectrometry based protein sequencing service. Combining both methods can provide comprehensive and accurate protein sequence analysis services for our customers. Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.

 

Analysis Workflow

1. Sample Preparation

Purifying the target protein to ensure high purity and appropriate concentration.

 

2. Coupling Reaction

Under alkaline conditions, the N-terminal amino acid of the protein reacts with PITC to form a phenylthiocarbamyl derivative.

 

3. Cleavage Reaction

The coupled product is cleaved under acidic conditions to produce a PTH amino acid.

 

4. Identification and Analysis

HPLC is used to separate and identify the PTH amino acid, determining its structure.

 

5. Cycle Repetition

Repeating the above steps for the remaining polypeptide to sequentially analyze the entire sequence.

 

Service Advantages

1. High Precision

Our Edman degradation method offers unparalleled precision in determining N-terminal amino acid sequences.

 

2. Customized Services

We provide customized protein sequence analysis services according to customer needs.

 

3. Professional Team

All the procedures are operated by our experienced professional team to ensure data reliability and accuracy.

 

Applications

1. Proteomics Research

Assisting researchers in analyzing protein structures and functions.

 

2. Biopharmaceuticals

Protein sequencing can be used for sequence confirmation and structural studies of protein drugs in drug development.

 

3. Basic Scientific Research

The information of protein sequence can support studies of important regions such as protein functional domains and active sites.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Protein Sequencing

4. Mass Spectrometry Images

5. Raw Data